它们是不同的扫描模式，emission sacn是发射光谱，设定激发波长Ex WL，扫描Em（设定Em start WL和Em End WL）；excitation scan则相反，是激发光谱，设定发射波长Em WL，扫描激发波长范围Ex start WL和Em End WL，。一般emission sacn比较常用，即已知激发波长Ex，扫Em发射光谱，因为Ex可从紫外光谱较轻...
(General Physics) the continuous spectrum or pattern of bright lines or bands seen when the electromagnetic radiation emitted by a substance is passed into a spectrometer. The spectrum is characteristic of the emitting substance and the type of excitation to which it is subjected. Compareabsorption ...
GFP TargetExpressed protein STED wavelength (nm)575 BibliographyCitations Laser line (nm)488 Standard filter setFITC Ex/Em (nm)488/510 Find products Resources BioProbes® Journalarticles Pushing the Limits of Fluorescence Microscopy: Fluorescent Probes for Sup...
To develop a rapid and nondestructive hygiene-monitoring system in meat-processing plants, plate count and ATP content on pork meat surfaces were quantitatively determined with particular attention to NAD(P)H fluorescence produced by microorganisms. An excitation (Ex)-emission (Em) matrix (EEM) was...
Table II.Excitation and Emission Maxima for Classes of CDOM Fluorophores Identified by EEMS Type of fluorophoreλex/λemLabeled Protein-like Tyrosine230, 275/305B Tryptophan230, 275/340T Humic-like UV-humic230/430 UV-humic260/400-460A
The acquisition of an EEM fluorescence spectrum involves the collection of sequential fluorescence emission (Em) spectra at successively increasing excitation (Ex) wavelengths. The Em spectra obtained are concatenated to produce a plot in which the fluorescence intensity is displayed as a function of Ex...
Fluorescence excitation-emission matrix spectroscopy (EEMs) combined with fluorescence regional integration (FRI) analysis was used to investigate the composition and transformation of humic acid (HA) and fulvic acid (FA) from landfill. The EEMs of HAs at different landfill ages were characterized by ...
A rich variety of processes driven by the above-bandgap excitation of ultrafast free-carrier photocurrents can also be investigated through the emitted THz waveforms, with\({{\bf{E}}}_{{\rm{THz}}}\left(t\right)=-\tfrac{\partial {{\bf{j}}}_{{\rm{f}}}{\partial t}\). The most...
The maximum emission peak (λem) is red-shifted from 400 nm to 530 nm along with the increase of λex. The excitation-dependent fluorescence is also one of the normal features of nonconventional luminophores in concentrated solution or crystals state9,25,46. Such excitation-dependent ...
Fig. 3. Normalized excitation spectra for a representative [ANS]/[HSA] =1 sample of each of the different polarization measurements and corresponding emission anisotropy of: (A) peak a, λem = 350 nm, intrinsic HSA fluorescence; (B) peak b, λem = 470 nm, ANS bound to HSA fluorescence...