The first was the selection of appropriate donor cell types for SCNT. Mouse cloning is usually performed using cumulus cells or immature Sertoli cells, which are collected from euthanatized animals. Such donor cells are not appropriate for our purpose of conserving the valuable genetic resources of...
Aggregation into 3D structures and extended culture on Transwell filters yielded organoids with defined tissue structures and distinct Sertoli cell marker expression. These studies provide insight into human gonadal development, suggesting that a population of precursor cells may originate from a more ...
Regeneration of the testis from pluripotent stem cells is a real challenge, reflecting the complexity of the interaction of germ cells and somatic cells. Here we report the generation of testicular somatic cell-like cells (TesLCs) including Sertoli cell-
1A). Immunostaining for CFTR at the same time frame confirmed that the expression of CFTR was increased in both germ cells and Sertoli cells as distinguished by the germ cell marker TRA98 (Suppl Fig. S1) [38]. Download: Download high-res image (508KB) Download: Download full-size image ...
Blood. 1993; 81: 3234-3241Crossref PubMed Google Scholar), and Sertoli cells in the testis (7Ito E. Toki T. Ishihara H. Ohtani H. Gu L. Yokoyama M. Engel J.D. Yamamoto M. Nature. 1993; 362: 466-468Crossref PubMed Scopus (257) Google Scholar,8Yomogida K. Ohtani H. Harigae ...
The amh gene, also called Müllerian inhibiting substance, is a member of the TGF-β family that is a key player in cell proliferation, differentiation and apoptosis [58]. It is secreted by Sertoli cells and is responsible for the regression of Müllerian duct during male fetal development in...
Welsh MJ, Wu W, Parvinen M, Gilmont RR (1996) Variation in expression of hsp27 messenger ribonucleic acid during the cycle of the seminiferous epithelium and colocalization of hsp27 and microfilaments in Sertoli cells of the rat. Biol Reprod 55:141–151 ...
the dimorphic expression may reflect thatGli1is essential for Leydig cell differentiation in the early fetal testis but are not essential in the ovary before after birth when theca cells are established63,64. In fetal mice testis, it has been shown that Sertoli cell-derived Hh-signalling induce...
Neurons that had been differentiated on Sertoli cells were positive for Pax2, En1, and AADC, midbrain-related markers, and negative for dopamine--hydroxylase, a marker of noradrenergic neurons. These Sertoli cell-induced dopaminergic cells can release dopamine when depolarized by high K+. Sertoli...
mouse embryonic stem cellsertoli cellintermediate mesodermin vitro differentiationSertoli cells (SCs) in the mammalian testes are well known as supporting cells of spermatogenesis, but have recently become an attractive source of cell therapy because of their capacity for immune modulation and trophic ...