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Live cells were gated with the forward scatter (FS-Peak-Lin) cutoff set at 390, with a gain value of 1.7. Values of mean fluorescence intensity (MFI) from approximately 20,000 cells per sample were used for analysis. Expression and purification of the dog β3AR The DNA encoding the β...
The ORF for CdtB was added downstream of PltB and PltA to be in-frame with the C-terminal His6 tag presented on pET28a+. This design allowed for the expression of all three genes by the induction of T7 promoter. Note: The resin should be stable for longer than a year. Proper ...