In this study, we describe a high-throughput adaptable screening method to detect inhibitors and compare their inhibition efficiency at nanogram levels, using a Competitive enzyme-linked immunosorbent assay (ELISA) based Trypsin Inhibition Assay (E-CTIA). We worked with four different inhibitors from ...
This chapter describes an ELISA-based protocol for 5hmC detection and quantification in genomic or in vitro modified DNA. It is based on the passive adsorption of DNA onto a solid polystyrene surface and the specific detection of 5hmC, which generates a measurable chemiluminescent signal, ...
Journal of Immunological MethodsBhattacharya D,Bhattacharya R,Dhar T K.A novel signal amplification technology for ELISA based on catalyzed reporter deposition... D Bhattacharya,R Bhattacharya,TK Dhar,... - 《Journal of Immunological Methods》 被引量: 73发表: 1999年 Bacterial-based systems for expr...
An ELISA-Based Assay for Detergent-solubilized Cellular β1,4-Galactosyltransferase Activity.acceptorsubstrateactivityPAP(GlcNAcβ)glycopolymerdifferentiationIn our previous paper [Oubihi et al. (1998) Anal. Biochem., 257, 169-175], we have shown that a polyacrylamide-derived synthetic glycopolymer ...
基于细胞的elisa,就是说它能测定完整的、经过固定的细胞中磷酸化蛋白及总蛋白水平,而无需裂解细胞. 作为一种全新的ELISA技术,它有两个最突出的优点:1)不需要抽提蛋白、包被微孔板:细胞直接在微孔板里培养,待检测的时候,将细胞固定在微孔板上并进行通透处理即可.这样就避免了抽提蛋白时,由于客观和主观上引起样品的...
Serum samples from 187 males 15 years of age and older representing four different exposure groups were tested for the presence of specific antibodies to Toxoplasma gondii, Echinococcus granulosus, and Trichinella spiralis antigen using an enzyme-linked immunosorbent assay (ELISA). The exposure groups ...
Genetic effects of ELISA-based segregation for control of bacterial kidney disease in Chinook salmon (Oncorhynchus tshawytscha). (English):We evaluated genetic variation in ability of Chinook salmon (Oncorhynchus tshawytscha) to resist two bacterial pathogens: Renibacterium salmonina... Hard,Jeffrey,J,...
The invention provides a PCR-ELISA (polymerase chain reaction enzyme-linked immunosorbent assay) based method for detecting a mycobacterium tuberculosis resistance gene. The method comprises the steps of: (1) designing 11 probes directed at rpoB gene mutation sites, and applying the probes on a 96...
A recently described procedure for the evaluation of the affinity of monoclonal antibodies [Friguet et al., J. Immun. Meth. 77, 305–319 (1985)] uses an ELISA system to determine the quantity of free antibody present in a mixture of antigen and antibody. However, an intact IgG may bind...
Genetic effects of ELISA-based segregation for control of bacterial kidney disease in Chinook salmon (Oncorhynchus tshawytscha) We evaluated genetic variation in ability of Chinook salmon (Oncorhynchus tshawytscha) to resist two bacterial pathogens: Renibacterium salmoninarum, the a... JJ Hard,DG Ellio...