The reaction was stopped after suitable color development by the addition of 2 M sulphuric acid and the absorbance at 450 nm was read using an ELISA Reader. Three parallel wells of each test were detected, and the mean of the absorbance from three wells were used to calcu- late the ...
The absorbance is measured on a microtiter plate reader. The intensity of the colour formed by the enzymatic reaction is directly proportional to the concentration of SHBG in the sample.A set of standards is used to plot a standard curve from which the amount of SHBG in patient samples and ...
•MicrotiterplatereaderwithsoftwarecapableofmeasurementatorintheELISATechnicalGuideavailableatthermoshercom. near450nm•Allowreagentstoreachroomtemperaturebeforeuse.Mixto •Platewasher–automatedormanual(squirtbolemanifoldredissolveanyprecipitatedsalts. dispenser,orequivalent) Prepare1XWashBuffer 1.Dilute16mLofWas...
Accordingly, the reaction mix from the first plate was transferred to a UV transparent 96-well plate, containing water to dilute the nucleosides to 0.1 mM concentration, and then the UV-transparent plate was read at 245 and 265 nm on a Synergy H1 hybrid Reader (Biotek). The reaction...
8.Determinetheopticaldensity(ODvalue)ofeachwellatoncewithamicro-platereader setto450nm. -19- AssayProcedureSummary -20- Calculationofresults Averagetheduplicatereadingsforeachstandardandsamples,thensubtracttheaverage zerostandardopticaldensity.Plotafourparameterlogisticcurveonlog-logaxis,with standardconcentration...
In order to quench the reaction, 50 μL 1 M H2SO4 was added, and the plate was analyzed within 10 min of the acid addition at OD450 subtracting OD655 (EnVision® plate reader). 2.4. AlphaLISA analysis 5μL of the neutralized reaction sample was transferred to a 384-well microtiter...
4.ELISA reader capable of reading absorbance at 450 nm 5.Absorbent paper or paper towel 6.Graph Paper Precautions and Disclaimer This product is for R&D use only, not for drug,household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling ...
Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis Protocol Outline Prepare all reagents, samples and standards as instructed in the manual. Add 100 µl of standard or sample to each well. ...
The reaction was stopped by the addition of 50 μL of 2 N H2SO4 (Sigma-Aldrich, St. Louis, MO, USA) and the OD was measured at 450 nm using a microplate reader (BioTek, VT, USA). All plate washing steps were performed with PBS (pH 7.2) containing 0.05% Tween 20 (PBS-T) ...
F-TESTO（自由测osterone）ELISA试验包（UNFI0105）产品说明书 Technical Manual F-TESTO (Free Testoterone) ELISA Kit (UNFI0105)•Catalogue Code: UNFI0105 •Competitive ELISA Kit •Research Use Only