(4) 洗涤 按照步骤(2)进行洗涤。 三、孵育: (5) 加入抗体偶联物溶液 加入抗体偶联物溶液 (Ab-HRP),使用与步骤(1)相同体积的溶液体积,在含 0.5%BSA的 0.05% PBST 缓冲液中进行两倍系列稀释 (至少三个稀释度)。每个稀释度做三次重复。封板后于室温孵育 1 小时。 (6) 洗涤 去除溶液,并使用 ELISA 洗板...
[22] Li M, An W, Wang L, et al. Production of monoclonal antibodies for measuring Avastin and its biosimilar by Sandwich ELISA. J Immunol Methods. 2019;469:42-46. doi:10.1016/j.jim.2019.03.013 [23] Dou Y, Lv Y, Zhou X, et al. Antibody-sandwich ...
[4] Lin A. Direct ELISA. Methods in Molecular Biology. 2015:61-67. doi: 10.1007/978-1-4939-2742-5_6 [5] Tips for Reducing ELISA Background. Biocompare. https://www.biocompare.com/Bench-Tips/122704-Tips-for-Reducing-ELISA-Background/. Pu...
Iqbal A, Farooq S, Jamal Y, et al. Detection of food allergens by ELISA and other common methods. Fresenius Environ. Bull. 2018;27(12): 8340-8346. Pandey AK, Varshney RK, Sudini HK, Pandey MK. An improved enzyme-linked immunosorbent assay (ELISA) based protocol using seeds for detection...
Mabtech is a Swedish Biotech company focused on ELISpot, FluoroSpot, ELISA, and FociSpot kits and instruments.
[1] Frey A, Meckelein B, Externest D, et al. A stable and highly sensitive 3, 3′, 5, 5′-tetramethylbenzidine-based substrate reagent for enzyme-linked immunosorbent assays[J]. Journal of immunological methods, 2000, 233(1-2): 47-56....
‹Pierce Protein Methods Overview of ELISA ELISA Development and Optimization Spike and Recovery and Linearity of Dilution Assessment Factors Affecting Signal Generation in ELISA ELISA Troubleshooting Guide ELISA Data Analysis Detergents for Cell Lysis...
[1] Frey A, Meckelein B, Externest D, et al. A stable and highly sensitive 3, 3′, 5, 5′-tetramethylbenzidine-based substrate reagent for enzyme-linked immunosorbent assays[J]. Journal of immunological methods, 2000, 233(1-2): 47-56....
Methods Biotin and hapten-labeled drugs are incubated with the patient serum sample to allow ADA to form a bridge complex with each drug conjugate. The reaction mixture is then added to a test strip with an anti-hapten capture zone which captures the mixed bridge complex. The bridge-complexed...
Methods for Detecting Protein–RNA Interactions Chromatin IP (ChIP Assays) Methods for Labeling Nucleic Acids Overview of Mass Spectrometry Quantitative Proteomics Sample Preparation for Mass Spectrometry Overview of Protein Labeling Biotinylation Metabo...