在Excel中使用曲线拟合方法进行ELISA结果分析,可以按照以下步骤进行操作: 将实验数据输入Excel表格中,其中包括标准品的不同浓度对应的吸光度值和待测样品的吸光度值。 选择数据区域,点击“插入”选项卡,选择“散点图”功能。将散点图插入到Excel表格中。 右键单击散点图上的数据点,选择“添加趋势线”选项。在弹出的...
可以使用Excel等软件进行计算。 2.方差分析:如果实验中有多个组别或处理,可以使用方差分析(ANOVA)进行组间比较。方差分析可以判断不同组别之间是否存在显著差异。 3.相关性分析:如果实验中有多个变量,可以进行相关性分析,判断变量之间的相关程度。可以使用Pearson相关系数或Spearman相关系数等方法进行计算。 4.统计显著性...
Written by Sean Mac Fhearraigh Seán Mac Fhearraigh PhDis a co-founder of Assay Genie. Seán carried out his undergraduate degree in Genetics at Trinity College Dublin, followed by a PhD at University College Dublin. He carried out a post-doc at the Department of Genetics, University of ...
Excel (sandwich-elisa.xlsm) Citations Qiang, L., Wang, J., Zhang, Y. et al. Mycobacterium tuberculosis Mce2E suppresses the macrophage innate immune response and promotes epithelial cell proliferation. Cell Mol Immunol 16, 380-391 (2019). https://doi.org/10.1038/s41423-018-0016-1 Species...
Excel (sandwich-elisa.xlsm) Citations Tebebi P., Burks S., Kim S., et al. Cyclooxygenase-2 or Tumor Necrosis Factor-a Inhibitors Attenuate the Mechanotransductive Effects of Pulsed Focused Ultrasound to Suppress Mesenchymal Stromal Cell Homing to Healthy and Dystrophic Muscle. Stem Cells 2015 ...
Modern readers have software bundles capable of exporting data in standard file formats, most often in Excel files. The immediate goal of ELISA data analysis is to calculate the concentration of the target molecule by using known standards. This procedure involves curve fitting and interpolation, ...
Alternatively; another data analysis software (e.g. the software provided with the plate reader) may be used as long as it supports the 4-parameter logistic curve fit model. d) Excel macro EMA31 calculation of DA concentration in Algal samples ...
Remarks: Sample collection and preservation methods can refer to the Excell Bio ELISA Kit instructions. The specific operation precautions can be communicated with the bioscientists at the company's research center. 4、Submit pre-experiment data ...
Log-log graph paper or computer and software for ELISA data analysis Protocol Outline Prepare all reagents, samples and standards as instructed in the manual. Add 100 µl of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4°C. ...
Alternatively; another data analysis software (e.g. the software provided with the plate reader) may be used as long as it supports the 4-parameter logistic curve fit model. d) Excel macro EMA31 calculation of DA concentration in Algal samples ...