实验原理 凝胶迁移或电泳迁移率实验(EMSA)是一种研究DNA结合蛋白和其相关的DNA结合序列相互作用的技术,可用于定性和定量分析。这一技术最初用于研究DNA结合蛋白,目前已用于研究RNA结合蛋白和特定的RNA序列的相互作用。 通常将纯化的蛋白和细胞粗提液和32P同位素标记的DNA或RNA探针一同保温,在非变性的聚丙烯凝胶电泳上,...
6. Electrophoretic mobility shift assays(EMSA):凝胶迁移滞后实验,是一种研究核酸与蛋白质相互作用简单、快速、敏感的方法,其基
什么是EMSA? 什么是EMSA(Electrophoretic Mobility Shift Assay)? 凝胶迁移或电泳迁移率实验(EMSA)是一种研究DNA结合蛋白和其相关的DNA结合序列相互作用的技术,可用于定性和定量分析。这一技 - 生科学姐于20231123发布在抖音,已经收获了9283个喜欢,来抖音,记录美好
Electrophoretic Mobility Shift Assay (EMSA) Service Electrophoretic mobility shift assay(EMSA, also calledgel retardation assayorgel shift assay) is anin vitromethod to detect the interaction between proteins and nucleotides. It is originally used to detect transcription factors, and is now further devel...
The gel electrophoresis mobility shift assay (EMSA) is used to detect protein complexes with nucleic acids. It is the core technology underlying a wide range of qualitative and quantitative analyses for the characterization of interacting systems. In the classical assay, solutions of protein and nucle...
中国试剂网3.9.197 凝胶迁移滞后实验(electrophoreticmobilityshiftassays,EMSA)基本原理 凝胶迁移滞后实验(electrophoreticmobilityshiftassays,EMSA)是近年发展起 来的研究核酸与蛋白质相互作用简单、快速、敏感的方法。目前已经成为转录因 子研究的经典方法。 其基本原理是蛋白质可以与末端标记的核酸探针结合,电泳时这种复合...
网络电泳迁移率实验;凝胶阻滞 网络释义
必应词典为您提供electrophoretic-mobility-shift-assay-EMSA的释义,网络释义: 凝胶电泳迁移实验;凝胶迁移试验;凝胶迁移阻滞技术;
Electrophoretic Mobility Shift Assays for Protein–DNA Complexes Involved in DNA RepairUV-DDBGlobal genomic repairNucleotide excision repairKuDNA-PKcsXRCC4/Ligase IVDNA-PKNonhomologous end joiningDouble-strand break repairV(D)J recombinationThe electrophoretic mobility shift assay (EMSA) can be used to ...
This fluorescence-based Electrophoretic Mobility Shift Assay (EMSA) Kit provides a fast, easy and quantitative method to detect both nucleic acid and protein in the same gel. This kit uses two fluorescent dyes for detection - SYBR Green E