The gel electrophoresis mobility shift assay (EMSA) is used to detect protein complexes with nucleic acids. It is the core technology underlying a wide range of qualitative and quantitative analyses for the cha
【题目】名词解释电泳迁移率变动分析(electrophoretic mobility shift assay,EMSA) 相关知识点: 试题来源: 解析 【解析】 电泳迁移率变动测定:是检测蛋白质和DNA序列体外相互结合的方法。蛋白质与特定寡 核苷酸探针结合,将增大其分子量,使其在凝胶中的电泳速度慢于游离探针,即表现为条带相对滞 后,从而判断蛋白质与...
实验原理 凝胶迁移或电泳迁移率实验(EMSA)是一种研究DNA结合蛋白和其相关的DNA结合序列相互作用的技术,可用于定性和定量分析。这一技术最初用于研究DNA结合蛋白,目前已用于研究RNA结合蛋白和特定的RNA序列的相互作用。 通常将纯化的蛋白和细胞粗提液和32P同位素标记的DNA或RNA探针一同保温,在非变性的聚丙烯凝胶电泳上,...
6. Electrophoretic mobility shift assays(EMSA):凝胶迁移滞后实验,是一种研究核酸与蛋白质相互作用简单、快速、敏感的方法,其基
Biochemical Assays and Intracellular Signaling Electrophoretic Mobility Shift Assay (EMSA) An electrophoretic mobility shift assay (also known as a gel shift assay) is used to determine if a protein is able to directly interact with a short, specific sequence of DNA. Before the experiment begins, ...
Biochemical Assays and Intracellular Signaling Electrophoretic Mobility Shift Assay An electrophoretic mobility shift assay (EMSA, also known as a gel shift assay) is used to determine if a protein is able to directly interact with a short, specific sequence of DNA. Before the experiment...
Q: What kind of data analysis and reporting does Profacgen provide with EMSA services? Resources References: Mansouri-Noori F.;et al. Electrophoretic mobility shift assays (EMSAs) for in vitro detection of protein-nucleic acid interactions.STAR Protoc. 2024;5(2):103128. ...
必应词典为您提供electrophoretic-mobility-shift-assay-EMSA的释义,网络释义: 凝胶电泳迁移实验;凝胶迁移试验;凝胶迁移阻滞技术;
凝胶迁移或电泳迁移率实验(EMSA)是一种研究DNA结合蛋白和其相关的DNA结合序列相互作用的技术,可用于定性和定量分析。这一技术最初用于研究DNA结合蛋白,目前已用于研究RNA结合蛋白和特定的RNA序列的相互作用。 通常将纯化的蛋白和细胞粗提液和32P同位素标记的DNA或RNA探针一同保温,在非变性的聚丙烯凝胶电泳上,分离复合物...
The utility model discloses a chemiluminescence EMSA (Electrophoretic Mobility Shift Assay) kit. A kit body is made of a hard plastic material, a drawer is arranged in the front of the kit body, a reagent pad made of hard plastic foam is arranged in the kit body, a groove A and a ...