We sought to document patterns of scarring highlighted by elastic tissue staining in primary cicatricial alopecia. We documented Verhoeff elastic van Gieson staining patterns in 58 routinely embedded (vertical) biopsy specimens of cicatricial alopecia. Patterns of fibrosis included perifollicular (wedge-...
The section (5 µm) were stained for Miller’s Elastica van Gieson stain to visualize the elastic fibers. Hematoxylin and Eosin (H&E) staining were additionally performed. Resulting histological slices were examined using a light microscope (Observer Z1, Carl Zeiss Microscopy GmbH, Jena, ...
Several histological stains have been adapted to visualize elastin in tissues, but the most commonly used is Verhoeff's Van Gieson stain (commonly abbreviated, VVG). VVG staining is quick and produces intense staining of elastic fibers. The VVG stain consists of two parts: Verhoeff stain consistin...
Collagen stainable by Van Gieson's method... K Jayasankar,VD Ramanathan - 《Indian Journal of Medical Research》 被引量: 13发表: 1999年 Connective tissue. VII. Changes in protein and hexosamine content of bone and cartilage of rats at different ages 1. Proteins of bone and cartilage from...
illustrate a control aorta and a treated aorta, respectively, following Verhoeff van Giesson staining, which stains elastin black. As can be seen the control aorta has very little elastin, while the treated aorta has retained elastin (shown by the black staining of the elastic fibers in the ...
Conventional staining technics for connective tissues, e.g., elastica stains, Van Gieson's picro-fuchsin, and trichrome methods, were introduced between 1880 and 1910, but their chemical mechanism and significance remained obscure. Interpretations of staining patterns were based on tradition rather than...
Petros MoustardasNikolaos P. E. KadoglouMichalis KatsimpoulasAlkistis KapelouzouNikolaos KostomitsopoulosPanayotis E. KarayannacosAlkiviadis KostakisChristos D. Liapis
Elastic fibersElderly cadaverVerhoeff van Gieson stainingPurpose To define the localization and configuration of the elastic fibers of the cricoarytenoid ligament (CAL) and their relationship with the cricoarytenoid joint (CAJ) capsule.Methods Twenty-four CAJs from twelve cadavers were analyzed using...
At both time points, the presence and extend of atherosclerotic plaque was confirmed with Elastica Van Gieson staining (Figure 2). Histological evaluation of the innominate artery plaque size and elastin content (western blot) were in good agreement with the imaging findings ...
Histopathological examination was performed by Elastica van Gieson staining, scanning electron microscopy, transmission electron microscopy, and ultra-high voltage electron microscopy. The number of pixels representing elastin fibers in computerized images was analyzed using Adobe Photoshop Version 2.0. Results...