答案:Sorbitol MacConkey 2. 在EHEC的检测中,PCR技术通常针对___基因进行扩增,以检测EHEC的存在。 答案:stx 三、简答题 1. 请简述EHEC的检测流程。 答案:EHEC的检测流程通常包括样品的收集与处理、选择性培养基的富集培养、分离培养、分子生物学检测(如PCR)以及血清学检测等步骤。 2. 为什么EHEC的检测需要使用选择...
coli among healthy cattle in Golestan province. Materials and Methods: Fecal samples were collected from 180 clinically healthy cattle in Golestan province. After primary enrichment, samples were streaked on sorbitol MacConkey agar supplemented with cefixime and potassium tellurite (CT-SMAC). Non-...
A commercial enzyme immunoassay system (EHEC-Tek) was compared with immunomagnetic separation followed by culture to cefixime–tellurite–sorbitol MacConkey agar (IMS/C) for detectingEscherichia coliO157 in artificially inoculated beef samples and naturally contaminated beefburgers. The EHEC-Tek assay used...