Can detect multiple targets and multiple alteration types simultaneously even with small amounts of input nucleic acids [44] : PCR-based NGS: 5–10 ng total DNA (including malignant and normal DNA); hybrid capture-based NGS: 100–200 ng RNA or DNA; whole-genome NGS: micrograms of DNA [10...
Clinicopathological features for these patients included a median age of 8 years [range 1–15 years] with a sex ratio M:F of 4:1; all tumours were located in midline brain structures (pons or thalamus) and were either unilateral or bilateral. All patients were treated with focal radiation t...