All data are expressed as mean ± S.E. RESULTS Chondrocyte-specific EGFR Activity Is Required for SOC Formation We first performed immunohistochemistry to examine whether epiphyseal cartilage expresses EGFR. As shown in Fig. 1A, panel a, the majority of epiphyseal chondrocytes, except most of the...
Normalization was done using the median of the ratio of the read count to the geometric mean of read counts across samples as implemented in DESeq [102]. Transfection by LNAs in vitro Tumor cells were plated in a complete growth medium in a six-well plate to reach 50–60% confluence. ...
Analysis of (B) RNAscope and (C) immunofluorescence staining of ALOX15B, data represented as mean dots/intensity per mm2 of the epidermis (N = 3). Gene expression (D) or Western (E) analysis of ALOX15B in keratinocytes treated with control or ALOX15B siRNA for 72 h, followed ...
After the participants sat quietly for at least 5 min, blood pressure measurement was completed with an electronic blood pressure monitor (GB3053–93) of the nondominant arm three times every minute to obtain the mean. Laboratory examinations After a fasting period of more than 10 h, blood (...
Data are representative of mean ± SEM from three independent experiments. e, f Naïve CD4+ T cells from WT mice were in vitro differentiated under Th9 polarizing conditions with or without 10 ng/ml IL-33 or 10 ng/ml TSLP for 3 days respectively; e qPCR analysis of Il9, ...
Values represent the mean ± S.D. of three independent experiments as -fold increases. E, HeLa cells were stimulated with the indicated concentration of 3 ng/ml EGF for 10 min. Chemical inhibitors were pretreated for 30 min before addition of EGF. Whole-cell lysates were separated by Zn2+...
Mean ± SD, n = 3. Asterisks (**) denote significant differences compared with the control (0 µm), P < 0.05. of the EGFR inhibitor gefitinib and the mTOR inhibitor everolimus in the single-drug group reached 10 μM. However, the drug concentration in the combination group was 0.5-...
(mean + S.E.M.) were calculated relative to the EGFR level at t = 0 and plotted over time. (Welch’s two-tailed t-test; *P < 0.05).CRepresentative PLA images testing the association between p-EGFR and RAB7 after 30-min EGF treatment of U-118 control and EMP3 KO ...
The results were presented as the mean of the relative tumour volume for each experimental group with the s.e.m. Statistical significance was evaluated by using the two-way repeated ANOVA. Flow cytometry. MDA-MB-231 (5 Â 105 cells per condition) expressing MT4-MMP-Flag (Truong et al, ...
level was correlated with TNBC tumour volume.EPDZK1 protein level was correlated with TNBC lymph node metastasis.FK-M curve of TNBC patients. TNBC patients were divided into high and low groups according to the median value of PDZK1 protein level. Data were presented as mean ± SD. ...