pLS-SV40-mP-EGFP载体价格质粒购买价格是1200元,pLS-SV40-mP-EGFP载体质粒图谱(Vector map),pLS-SV40-mP-EGFP (addgene 137724)载体序列(Sequence)见下文,载体质粒抗性为氨苄霉素,质粒大小为8136 bp。pLS-SV40-mP-EGFP载体是哺乳细胞表达,慢病毒载体质粒。
EGFP在大肠杆菌E.coli中的表达与检测.EGFP在E.coli中的表达与检测 3组 contents 一二三四五 背景介绍实验流程实验步骤预期结果参考文献 第几章 一、背景介绍 1、绿色荧光蛋白（GFP）2、质粒3、大肠杆菌表达载体及表达系统4、SDS-PAGE原理及蛋白分离与检测 一1-绿色荧光蛋白 1.1发现 下村修 马丁-查尔菲 钱学健 ...
The MCS in pIRES2-EGFP is between the immediate early promoter of cytomegalovirus (PCMV IE) and the IRES sequence. SV40 polyadenylation signals downstream of the EGFP gene direct proper processing of the 3' end of the bicistronic mRNA. The vector backbone also contains an SV40 origin for ...
4、nm; emission maximum = 507 nm.) pEGFP-N3 encodes the GFPmut1 variant (4) which contains the double-amino-acid substitution of Phe-64 to Leu and Ser-65 to Thr. The coding sequence of the EGFP gene contains more than 190 silent base changes which correspond to human codon-usage prefe...
The RNA interference (RNAi) is a powerful tool to silence gene expression post-transcriptionally, and causes the degradation of an mRNA containing the same sequence. In this present study, an alternative approach was used to in vitro synthesize enhanced green fluorescent protein (EGFP) specific ...
mRNA expression of mLumin and 载体包括Rluc基因和一个多克隆位点;另一个载 eGFP iII HEK293T cells tansfected with 体为Fluc基因,用作转染和数据标准化的内 psiCHECK-2-eGFP.mLumin 参¨4。这种双荧光素报告系统在使用时的缺点显 2.3.2荧光显微镜检测psiCHECK一2一eGFP—mLumin ...
pCMS-EGFP is a mammalian expression vector that allows you to express your gene of interest at high levels by cloning it into a multiple cloning site (MCS) downstream of the immediate early promoter of cytomegalovirus (PCMV IE). The intervening sequence (IVS) between PCMV IE and the MCS is...
(PCMVIE)andtheEGFPcodingsequences.GenesclonedintotheMCSwillbeexpressedasfusionstotheNterminusofEGFPiftheyareinthesamereadingframeasEGFPandtherearenointerveningstopcodons.SV40polyadenylationsignalsdownstreamoftheEGFPgenedirectproperprocessingofthe3'endoftheEGFPmRNA.ThevectorbackbonealsocontainsanSV40originforreplication...
betweentheimmediateearlypromoterofcytomegalo(PCMVIE)andtheIRESsequence. SV40polyadenylationsignalsdownstreamoftheEGFPgenedirectproperprocessingofthe3end ofthebicistronicmRNA.ThevectorbackbonealsocontainsanSV40originforreplicationin r mliancellpressingtheSV40Tantigen.Aneomycin-cassette(Neo),consistingof ...
The EGFP DNA sequence of the recombinant pHL1003-GFP was consistent with the EGFP sequence in the pEGFP-N1 plasmid by DNA sequencing analysis. After 72 h transfection , the GFP gene at downstream of the bi-cistronic mRNA was able to be expressed effectively, and the strong fluorescence of ...