Acetoin is widely used in food and cosmetic industry as taste and fragrance enhancer. For acetoin production in this study, Saccharomyces cerevisiae JHY605 was used as a host strain, where the production of ethanol and glycerol was largely eliminated by
16. The 20-nucleotide sequence at the 5′ or 3′ end of the gRNA specifies the target site through RNA-DNA hybridization17,18,19. As a result, targeting different genes using CRISPR/Cas can be more easily achieved through
Due to its inexpensive fermentation requirements, rapid proliferation ability and stable high-level expression,Escherichia coli(hereafterE. coli) has become the mainstay of RP expression among prokaryotic expression hosts [5]. As early as the 1970s,E. coliwas applied in the production of clinical ...
When minimum efficient scale can be achieved with a small amount of production, many companies can operate efficiently and compete in an industry, such as restaurants. However, if it takes a high level of production output to achieve minimum efficient scale, fewer companies can operate in the in...
As a lower 3C activity might be compatible with an enzyme level sufficient to allow P1-2A expression and subsequent cleavage, expression cassettes were designed to reduce 3C activity. Two approaches were used: site-directed mutagenesis of 3C, based on the three-dimensional structure of the enzyme...
A higher level of scientific and technological informatization must be achieved, so as to facilitate the early perception, identification, warning and release of hazards and risks. Furthermore, a support system of emergency supplies, emergency communications, command platforms, equipment allocation, ...
China is fully tapping into the potential of digital technology in its innovative practice of ecological protection and green development. China will make its development greener and more prosperous as it pursues high-level ecological protection and modernization of ecological governance....
EL SEGUNDO, Calif.— January 2023 — EPC announces the introduction of the EPC21701, a laser driver that monolithically integrates an 80 V, 40 A FET with gate driver and 3.3 logic level input into a single chip for time-of-flight lidar systems used in robotics, surveillance systems, and ...
SSF also keeps the free sugar level continuously low, which counteracts bacterial contamination. On the other hand, SSF has to be conducted at the fermentation temperature of yeast (±35°C), which is considerably lower than the optimal temperature of the lignocellulolytic enzymes (±50–60°C)...
data that should be allocated per device to hide proprieties of the original input. We formulate this methodology, as an optimization, where we establish a trade-off between the latency of co-inference and the privacy-level of data. Next, to relax the optimal solution, we shape our approach...