In laboratory science, EDTA is also used for: Scavenging metal ions: in biochemistry and molecular biology, ion depletion is commonly used to inactivate metal-dependent enzymes which could damage DNA or proteins Complexometric titrations. Buffer solutions. Determination of water hardness. Used in...
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分组:每一次实验分为不染组、单染Annexin V组、单染PI组以及PI和Annexin V双染组,处理组按从低到高进行双染; 染色:用PBS把4×binding buffer稀释到1×缓冲液,吸净离心管残余的PBS后,每管加入100μL的1×的binding buffer,用移液枪吹打细胞使细胞充分重悬,避光条件下加入染料。不染组不加,单染组加Annexin ...
calcium bindingkineticsslow buffertransient currentStudying the currents in perfused cells or fragments of their membranes with voltage-gated calcium channels and Ca~2+-dependent ion channels, it is ofter expedient to use a buffer that binds Ca~2+ slowly. for this purpose, we propose EDTA in ...
固定前需要先将细胞与Annexin V-FITC进行孵育,并用binding buffer洗掉未结合的Annexin V-FITC。因为固定过程中细胞通透性增加会产生细胞碎片,可以和Annexin V结合,对结果产生干扰。 4) 如果样品来源于血液,请务必除去血液中的血小板。因为血小板含有PS,能与Annexin V结合,从而干扰实验结果。可以使用含有EDTA的缓冲剂并...
Buffer solutions. Determination of water hardness. EDTA may be used as a masking agent to remove a metal ion which would interfere with the analysis of a second metal ion present Ananticoagulantin medical and laboratory equipment. A preservative (usually to enhance the action of another preservativ...
It is also used to prepare buffer solutions with known metal ion concentrations.Packing: in 25KG bag Company Profile Exhibition Send your message to this supplier *From: *To: *Message: Enter between 20 to 4,000 characters. Send This is not what you are looking for? Post a ...
○ = marker in water or MES buffer; ▪ = autoclaved clarified rumen fluid 1 or 3; ▴ = autoclaved clarified rumen fluid 2 or 4. The CoEDTA preparations in MES and in pH-adjusted ACRF differed in pH (P < 0.01; SED = 0.004; Figure 1E). However, the differences were small, ...
Dankert and Schut, “The antibacterial activity of chloroxylenol in combination with ethylenediaminetetra-acetic acid,” Journal of Hygiene, 76(1):11-22, 1976. ;76(1):11-22, 1976. Davis and Iannetta, “Influence of serum and calcium on the bactericidal activity of gentamicin and carbenicillin...
Growing interest has been paid to preparation of nitridotechnetium complexes, because the oxidation state of technetium in nitridotechnetium complexes ranges from +5 to +7 and the nitridotechnetium core (TCEN) is stable toward hydrolysis and ligand exchange reactions1'4).The formation of nitrido...