Numbering on the x-axis is relative to the ATG start codon for each gene, with sequence upstream indicated by a minus (−) symbol and sequence downstream indicated by a plus (+) symbol. The green vertical line indicates the Cas9 or TevCas9 cleavage site, while the shaded rectangle ...
Numbering on the x-axis is relative to the ATG start codon for each gene, with sequence upstream indicated by a minus (−) symbol and sequence downstream indicated by a plus (+) symbol. The green vertical line indicates the Cas9 or TevCas9 cleavage site, while the shaded rectangle ...
All plots were generated in R using Seurat, ggplot2 (version 3.3.2), ggpubr (version 0.2.4) and pheatmap (version 1.0.12). CRISPR gRNA enrichment analysis For Jurkat gRNA enrichment analysis, CRISPR gRNA enrichment reads were counted; dual gRNAs were paired in silico from paired-end reads...
The resulting per-base score (y-axis) along each sequence read was plotted using the ggplot and ggjoy packages in R to produce Fig. 1a and Additional file 1: Figure S3. Total pairwise identity scores were used to create Additional file 1: Figure S2d,e. Background mismatches resulting ...
Density and rank plot analyses were performed in R version 3.5.0 (2018-04-23) and original plots were produced using the ggplot2 package. When required, sgRNA efficiency values were obtained from Rule Set 2 on-target scores using the GPP portal suite for sgRNA design (Table S3). Unless ot...
(CAR) atTRACwith Recursive Editing in CD4 + T cells. Left y-axis corresponds to the percentage of cells expressing T cell receptor α/β (TCR) and the right y-axis to the percentage of cells expressing the CAR. g526 was a previously identified high performing gRNA30. Individual ...
(on-target) loci are labeled as OG, while predicted off-target sites for primary gRNAs are labeled as OG_OT, and predicted off-target sites for secondary gRNAs are labeled as DT_OT on the y axis. The label on the x-axis indicates which gRNA the cells were transfected with; the ...
stages of library construction as a function of what percentage of the reads (Y) in each sample are attributed to what percentage (X) of the top barcodes/features in each sample, where the barcodes/features are sorted from highest to lowest abundance from left to right on the x-axis. Th...
Navega Therapeutics and Engine Biosciences. The terms of these arrangements have been reviewed and approved by the University of California, San Diego in accordance with its conflict of interest policies. Y.S. is an employee of Shape Therapeutics. D.K. is now an employee of Shape Therapeutics....
a,bRNA-seq of the pectoral fins. Minus-Average (MA) plots with the average gene expression levels on theXaxis and log2fold-change in expression between the sexes inO. woworae(a) or between O. woworaemales andO. asinuamales (b) on theYaxis. Each dot represents a single gene with dif...