(2023) clarified the function of E2 and its homologous genes E2-Like a and E2-Like b [42]. They designed a single and double mutants of E2 and its homologous genes through CRISPR/Cas9 knock out method to invest
in which the cultivation of a male sterile line is the key to the success of cross-breeding. CRISPR/Cas9 systems have been widely used in target-site genome editing, whereas their application for crop genetic improvement has been rarely reported. Here, using the CRISPR/Cas9 system, we induced...