我最近想用edc/nhs体系将一个含有羧基的药物接枝到氨基表面上，但是该药物目前比较好溶的溶剂只有dmso和...
请教各位大虫,我最近想用edc/nhs体系将一个含有羧基的药物接枝到氨基表面上,但是该药物目前比较好溶的溶剂只有dmso和甲醇,不知道这两个溶剂可不可以使用,如果有副产物的话该怎样除去? 展开 0评论 +关注 共2个回答 辞唱—,设备工程师 2019-01-02回答 我也是将含有羧基的样品接支到氨基的表面的,不过我用的...
The reaction was allowed to take place for 4 h at particles were fixed on the stub with a double-sided sticky tape and then coated 37 C. After centrifugation, the mixture was washed with DMSO to eliminate with platinum layer, which was carried out by an Auto Fine Coater (JEOL, un-...
NHS was added in an EDC:NHS ratio of 4:1. After crosslinking with a reaction time of 4 h the samples were washed twice with 0·1 m Na 2 HPO 4 for 1 h and 4 times with deionized water for 30 min. Finally, collagen was dried in vacuum. 2.3 Characterization of collagen cross...
In addition, a lack of a carboxyl group or an amino group would cause failure in crosslinking. Table 1. Reaction specifications of series molar ratio hydrogels. HydrogelMolar ratio of PGA:PL:EDC:NHS–COOH of PGA (mol/L)–NH2 of PL (mol/L)EDC/NHS (mol/L)Crosslink PGA 1:0:0.25:...