Ectoine is overproduced by Halomonas salina DSM 5928, an ectoine-secreting strain, in which the synthesis of ectoine is not limited by its intracellular threshold concentration. In order to explain the mechanism of secretion of ectoine, the response to NaCl stress, and the release and uptake ...
The author introduced the advantages and shortcomings of chemical and enzymatic synthesis of uridine diphosphate glucose. 介绍了化学和酶法合成尿苷二磷酸葡萄糖(UDPG)及酶法合成13C标记UDPG的方法的优缺点及研究进展。 更多例句>> 3) enzymatic synthesis 酶促合成 1. In this article, We report a new ...
The base sequence of a DNA coding for an ectoine synthetase has been determined by isolating a gene coding for an enzyme participating in ectoine synthesis in the form of a DNA with about 4.2 kilobase pairs obtained by cleaving a Halomonas KS-3 strain gene DNA with restriction enzymes EcoRI...
Ectoine synthesis is carried out by the process of three genes: ectABC. The ectA gene codes for diaminobutyric acid acetyltransferase; ectB codes for the diaminobutyric acid aminotransferase and ectC codes for ectoine synthase.Ectoine is synthesized from aspartate-semialdehyde, the central intermediate ...
To produce PhaP in high level, the native PHA synthesis pathway was blocked by deleting phaC gene. The phaP and T7-like expression system was integrated into H. bluephagenesis genome. The chromosome expression system produced 19% PhaP in total cellular soluble proteins with a yield of 1.86 g...
摘要:以耐盐菌SL07为生产菌.在30L发酵罐中合成ectoine。首先确定最佳诱导培养基.并在发酵过程中调节pH可提高ectoine合成量。在pH恒定条件下加盐.合成量降低1O;加蛋白胨,台成量提高43。发酵后的菌体可重复诱导培养.ectoine合成量比第1次高出40。中图分类号:Q935文献标识码:ASynthesisofectoinebyusinghalotolerant...
1Introduction to ectoines: natural occurrence, relevance and synthesis in prokaryotes and eukaryotes (protists) Ectoinesare part of the compatible solute family. These compounds are highly water soluble, mostly electrically neutral (physiological pH), small organic molecules, which protect cells and mac...
中图分类号:@935 文献标识码:A Synthesis f ect ine by using hal t lerant bacteria SLo7 YANG Jin-long 1 ,LANG Ya-jun 1,2 ,WANG Yue 1 (1. SchooI of BioIogicaI & Foodstuff Engineering,DaIian nstitute of Light ndustry,DaIian 116034,China; 2. CoIIege of EnviromentaI Science and Enginee...
design the production routes to ensure that we have been able to use biological, enzyme and water-phase green synthesis methods during the large production process to ensure that our health dietary supplements is truly high-quality ,green &environmental friendly, which are truly good and health to...
Cloning and Characterization of Ectoine Synthesis Genes and Cloning of Glutamine Transporter Genes from Halobacillus Dabanensis D-8～T; 达坂喜盐芽孢杆菌D-8～T四氢嘧啶合成基因的克隆、功能表达和谷氨酰胺转运蛋白基因的克隆 3. Optimization of Fermentation Conditions for Ectoine Synthesis from Moderately Ha...