In the current research, ectoine biosynthesis gene cluster (ectABC) in Bacillus clausii NIOT-DSB04 was expressed heterologically in E. coli M15 (pREP4). RP-HPLC resolved several fractions of the purified recombinant product, one of which had been confirmed as ectoine. The recombinant ectoine ...
[Objective] The aim of this study was to identify ectoine biosynthesis genes and analyze their function in Streptomyces Act12 by molecular biology methods... 高波,马怡茗,洪煜,... - 《西北农林科技大学学报(自然科学版)》 被引量: 0发表: 2017年 中度嗜盐菌Halomonas sp.TA-4产四氢嘧啶发酵条件...
Cloning of the ectoine biosynthesis gene ectABC from Halomonas sp. BYS-1 and salt-induced expression in Escherichia coli. Acta Microbiologica Sinica, 46 (1): 28～32 (in Chinese)He J, Huang X, Gu LF, Jiang JD, Li SP. Cloning of the ectoine biosynthesis gene ectABC from Halomonas sp....
Ectoine biosynthesis is regulated at the level of transcription and enzyme activity. Transcriptome analysis of H. elongata revealed an increase of mRNA from ectA, ectB, and ectC with increasing sodium chloride concentration from 0.6% (0.1 M) to 12% (2 M) NaCl. Similar results were obtained ...
(Fig. 1). The first step of ectoine biosynthesis is the transamination ofl-aspartate-β-semialdehyde tol-2,4-diaminobutyric acid (DABA)viaDABA aminotransferase (EctB). Subsequently, DABA is acetylated to N-γ-acetyl-l-2,4-diaminobutyric acid (ADABA) by the acetyltransferase EctA. Finally, ...
Mustakhimov II, Reshetnikov AS, Khmelenina VN, Trotsenko YA (2009) EctR—a novel transcriptional regulator of ectoine biosynthesis genes in the haloalcaliphilic methylotrophic bacterium Methylophaga alcalica . Dokl Biochem Biophys 429:305–308. : 10.1134/S1607672909060052...
Construction and characterization of an NaCl-sensitive mutant of Halomonas elongata impaired in ectoine biosynthesis - Go虉ller, Ofer, et al. - 1998 () Citation Context ...peron from B. pasteurii (DSM 33 T ), a PCR strategy with degenerate primers was used. To design the primers for the...
The implementation of a mutant aspartokinase enzyme ensured efficient supply of L-aspartate-beta-semialdehyde, the precursor for ectoine biosynthesis. We further engineered the genome of the basic C. glutamicum strain by integrating a codon-optimized synthetic ectABCD gene cluster under expressional ...
(1985). Biosynthesis: P. Peterset al.,FEMS Microbiol. Lett.71, 157 (1990). Calorimetric analysis of biosynthesis efficiency: T. Maskow, W. Babel,Biochim. Biophys. Acta1527, 4 (2001). Chromatographic determn: V. Riiset al.,Anal. Bioanal. Chem.377, 203 (2003). Osmoprotection inE. ...
Analysis of the transcriptional levels revealed that supplementation with ammonium sulfate enhanced the metabolic flux towards the biosynthesis of ectoine. Furthermore, by optimizing the copy number of ectA, ectB, and ectC, the recombinant E. coli ET11 (ectA:ectB:ectC = 1:2:1) produced 12.9 g...