We examined the role of the plasminogen activator/plasmin system in extracellular matrix (ECM) degradation by human mesangial cells cultured on thin films of 125I-labeled ECM (Matrigel). ECM degradation (release of 125I into the medium) was dependent on exogenous plasminogen, proportional to the ...
The proteasome degrades many short-lived proteins that are labeled with an ubiquitin chain. The identification of phosphorylation sites on the proteasome subunits suggests that degradation of these substrates can also be regulated at the proteasome. In yeast and humans, the unstructured C-terminal regi...
Histoblasts are labeled by driving nls-GFP expression in this tissue. Anterior and posterior nests are outlined in green and red, respectively. Note that the nests are sometimes split at the end of larval development (0 hAPF), as is the case here for the posterior nest. Scale bars, 50 ...
The circled region labeled “3DC1” defines the cell group of interest for further analysis. (B) Demonstration of the gating for cleaved PARP1( cPARP1) and γ-H2AX in sham RT (left) and RT 16 Gy (right) arms for normal cultures at 24 h. Radiation-induced biomarkers of interests could...
To better understand the metabolic consequences of complex I inhibition by mdivi-1 and rotenone, we used a fluorescently labeled deoxy-glucose analog, 2-NBDG, to track glucose uptake in floating and adherent cells. In MDA-MB-231 cells, AM and AR increased 2-NBDG uptake compared to AC cells...
(2) the enzyme labeled antigen or antibody (combination); (3) the substrate of the enzyme; (4) negative control products and positive control (qualitative determination), reference standard and control sera (quantitative); (5) dilution conjugates and specimen; (6) the washing liquid, in type ...
Two-dimensional gel electrophoretic analysis of [35S]methionine-labeled cells revealed a dramatic decrease in vinculin, alpha-actinin, and actin synthesis in cells grown on ECM, as compared to cells grown on plastic, while the synthesis of the tubulins and of the intermediate filament protein ...
End-organ regional blood flow (RBF) was quantified at study endpoints using fluorescently labeled microspheres. Before treatment, IHF calves had an ejection fraction (EF) of 33 ± 2% and left ventricular end-diastolic volume of 214 ± 18 ml with cardiac cachexia (0.69 ± 0.06 kg/day). ...
In order to validate the metabolic activity and evaluate the suitability of the hollow micro hybrids as a possible drug delivery system, the cellular uptake and cytotoxicity of FITC-labeled hollow spheres in vitro on H9C2 cardiomyoblast cells was investigated. For the uptake studies, H9C2 cardiomyob...
Sometimes, a college music student will say, “I don’t want to be labeled. Don’t even call it jazz; it’s all beyond category.” I get it, but at the same time, any single phrase you can play on an instrument has a heritage, so what lineage are you in? And if you know you...