Sorted Treg cells were pre-activated with anti-CD28 antibody and IL-2 and plated onto 96-well plates pre-coated with anti-CD3 antibody. Tconv cells were labeled with carboxyfluorescein succinimidyl ester (CFSE) and co-cultured with Treg cells at a 50:1 ratio. After 64 h, cells were ...