(1999) Sigma M, an ECF RNA polymerase sigma factor of Bacillus subtilis, is essential for growth and survival in high concentrations of salt. Mol Microbiol 32: 41-50.Horsburgh, M.J. and Moir, A. (1999) Sigma M, an ECF RNA polymerase sigma factor of Bacillus subtilis 168, is essential...
Here, we report crystal structures of transcription initiation complexes containing Mycobacterium tuberculosis RNA polymerase (RNAP), M. tuberculosis ECF σ factor σL, and promoter DNA. The structures show that σR2 and σR4 of the ECF σ factor occupy the same sites on RNAP as in primary ...
Regulation of antimycin biosynthesis by the orphan ECF RNA polymerase sigma factor σAntA DOI: 10.7717/peerj.253 Ryan F. Seipke,Elaine Patrick,Matthew I. Hutchings Keywords: Streptomyces,Antibiotics,Secondary metabolites,Actinomycetes,Gene regulation,ECF sigma factor,Antimycin Full-Text Cite this paper...
The expression of genes in Corynebacterium glutamicum, a Gram-positive non-pathogenic bacterium used mainly for the industrial production of amino acids, is regulated by seven different sigma factors of RNA polymerase, including the stress-responsive ECF-sigma factor SigH. The sigH gene is located in...
Bacterial RNA polymerase employs extra-cytoplasmic function (ECF) σ factors to regulate context-specific gene expression programs. Despite being the most abundant and divergent σ factor class, the structural basis of ECF σ factor-mediated transcriptio
3.实时荧光定量聚合酶链反应 (real-time quantitative polymerase chain reaction,real-timePCR) 技术检测SP-BmRNA和 TGF-β1mRNA 表达:按照 Trizol试剂盒(美国Invitrogen公司)步骤提取纯化总 RNA,逆转录反应按照试剂盒说明书(北京全式金...