Briefly, DNA was extracted from fresh frozen tissue using the Invisorb Spin Forensic kit (Stratec Biomedical AG, Germany). Amplification of IGHV regions by polymerase chain reaction was performed using VH framework region 1 consensus family specific primers (VH1-VH6), FR1C, and JH primers. ...
The relatively constant urine EBV DNA concentrations of the two healthy subjects using the two different-sized PCR assays suggests that the EBV DNA molecules detected in their urine were relatively intact when compared with the urine EBV DNA detected in the urine of the NPC patients. One possible...