The findings of this study could be used for evaluating the potential impact of nanoscale biochar to earthworms and guiding biochar-augmented vermicomposting. Graphical AbstractHighlights 1. The addition of 1–2 mm biochar resulted in a 1.9-fold greater reduction in organic matter compared to the...
Many studies report the effects of UV radiation on several organisms40,41,42. In particular on earthworm, Chuang et al. in 2006 reported that the earthworm,Amynthas gracilistolerate UV-A, dies when exposed to UV-B, and shows lower tolerance thanMetaphire posthumaspecies when exposed to UV-B...
The precipitate was collected and dried in lab temperature for the dialysis of enzyme. 2.4. Purification of CMCase The crude enzyme was blended with 100 mL of 0.2 M citrate phosphate buffer at pH 7.0 to concentrate the protein sample. After the dialysis with dis·H2O, 50 mL of enzyme ...
We did not correct earthworm biomass for potential bias caused by the gut contents. The density and biomass of earthworms sampled by the hand-sorting method was converted to an area of 0.25 m2. Then, for each of the plots, we calculated the area-weighted sum of the number and biomass of...