“What she ultimately might want, versus what she knows she can get done as an effective leader, are two different things,” said Ballentine, who co-sponsored anti-abortion legislation with Haley when she served in the state legislature. “And she’s not going to mislead the public and ma...
Cells were disrupted with an ultrasound Vibracell 75042 sonicator (Fisher Bioblock Scientific) for 35 sec (7 cycles of 5 sec pulse and 5 sec pause). The extracts were then ultracentrifuged for 1h at 100,000 g at 4°C to remove cellular debris. Protein concentration from the supernatants ...
2.5 M thiourea, 50 mM DTT, 25 mM spermine, and 5% CHAPS, and containing a protease inhibitor cocktail (Complete Mini) from Roche (1 tablet per sample). Cells were disrupted with an ultrasound Vibracell 75042 sonicator (Fisher Bioblock Scientific) for 35...