Therefore, this study aims of isolating hemolysis E.coli through the molecular detection of the hemolysin gene and determining the effects of gamma (cobalt-60) radiation on this gene and resistance these of isolates to the antibiotics under study. Forty isolates of E.coli are isolated depending ...
All of thepotential hemolysin encoding genes were characterized in silico.These geneswere cloned and expressed in Escherichia coli.The hemolytic activities of theexpressed proteins were assayed observing the hemolysis on sheep blood agarplates.Sphingomyelinase activities of the hemolysin candidates were ...
coli are grown in blood agar showing no signs of hemolysis. From literature, the enterohemolytic phenotype is typical of the human pathogenic Shiga-toxin producer E. coli (STEC) strains, such as E. coli O157:H7 [27,28]. For the on-site implementation of novel and efficient pathogen ...
Jansen: A new microbiological assay of thiamine and thiaminepyrophosphate in 0,2 ml of blood with a mutant of Escherichia coli. Int. Z. Vitaminforsch. 27, 279–284 (1957). CAS Google Scholar Johnson, B. C., and P. H. LIN: Nicotinic Acid Metabolism. I. The Use of Paper ...
coli MreB protein. The 9-residue peptide (C-terminal amide) and its N-terminal acetylated analog displayed broad-spectrum activity, killing Gram-negative bacteria, Gram-positive bacteria, and fungi. Extension with a tryptophan residue at the N-terminus drastically improved the activity of the ...
851 EXERCISE-INDUCED HEMOLYSIS IN SICKLE CELL DISEASE. • Orah S. Platt, Samuel E. Lux, and David G. Nathan. Division of Hematology and Oncology, Harvard Medical. School, Children's Hospital, Boston, MA. Investigation of a patient with sickle cell disease (SS) and hemoglobinuria led us ...
We also investigated the cytotoxic effects of the ligand and its metal derivatives on human red blood cells (HRBCs) by determination of their hemolysis rate and by application of the DAPI staining method. 2. Results and Discussion 2.1. Synthesis of the Ligand and Metal Derivatives The multi-...
geraniol loaded in LNPs, which would improve the efficacy of colistin againstE. coliand, in particular, MCR-1 transconjugants. Then, the effect of the combination onE. coliinner membrane permeabilisation was evaluated using propidium iodide (PI) uptake and compared to human red blood cells ...
Blood agar medium with dialysis membrane mounted between two layers of agar was applied to study the haemolytic activity of 28 strains of Serratia marcescens. Two kinds of lytic substances differing with their ability to pass through dialysis membrane were found. Haemolytic activity was not detected ...