(1) 绿色荧光报告菌株文库,用来检测大肠杆菌启动子活动; (2) 包含超过1900个启动子,背景菌株为:E. coli K12 strain MG1655; (3) 在全基因组范围内检测准确的启动子活动; (4) 在质粒水平上,所挑选的DNA区域很容易突变,能够验证启动子区域cis-regulatory位点。 大肠杆菌标记ORFs文库 (E. coli Tagged ORFs) ...
Therefore this strain (or at least th 54、e version obtained from the E. coli Genetic Stock Center) does NOT appear to be lacIq. According to Barry Wanner, this is an unexpected result. -Reshma 13:19, 5 May 2005 (EDT) We have now confirmed that BW25113, BW25141, and BW26434 are...
The recombinant plasmid DNA was transformed by electroporation into an expression host strain E. coli K12 W25113 using a Bio-Rad GenePulser. Transformed cells were plated on LB Agar plates with 10 μg/mL tetracycline and grown overnight at 37 °C. Single colonies were picked and inoculated in...
(Lucigen) o 3.25 E. coli K12 ER2738 (NEB) o 3.26 ER2566 (NEB) o 3.27 ER2267 (NEB) o 3.28 HB101 o 3.29 HMS174(DE3) o 3.30 High-Control(tm) BL21(DE3) (Lucigen) o 3.31 High-Control(tm) 10G (Lucigen) o 3.32 IJ1126 o 3.33 IJ1127 o 3.34 JM83 o 3.35 JM101 o 3.36 JM103 ...
E.coligenotypes大肠杆菌基因型手册 From OpenWetWare 1 Nomenclature Abbreviations 2 Methylation Issues in E. coli 3 Commonly used strains 3.1 AG1 3.2 AB1157 3.3 BL21(AI) 3.4 BL21(DE3) 3.5 BL21 (DE3) pLysS 3.6 BNN93 3.7 BW26434, CGSC Strain # 7658 3.8 C600 3.9 C600 hflA150 (Y1073, BN...
coli BL21 [27] strain (Supplementary Table 1). Each insert was cloned into the SapI site of the FX initial cloning vector (pINITIAL) [22] to obtain pINIT_emrA, pINIT_emrB, and pINIT_tolC. The inserts were sequenced for verification (Microsynth). The FX-expression vector p7XC3RH_emr...
concentration18. ΔcyaA,ΔcpdA,ΔmalTΔptsGstrains were constructed by transducing the appropriate deletion into NCM3722 from the Keio knockout collection (derived from the BW25113 strain59) by P1 transduction. Growth rate and promoter activity measurements ...
The E. coli insertional mutant strains JW2308–4 (ΔubiX), JW5713–1 (ΔubiC), and the respective wild type BW25113 were obtained from National Bio Resource Project (E. coli strain) (11), and AN66 was obtained from Coli Genetic Stock Center (12). The presence of the point mutation ...
coli BW25113 using the plasmid pBAD. Indeed, in the presence of arabinose, tyrosol failed to inhibit biofilm formation in only the ybfA-overexpressing E. coli strain, whereas FC inhibited biofilm formation in the ybfA-overexpressing E. coli strain regardless of the presence of arabinose (Figs...
(2) 包含超过1900个启动子,背景菌株为:E. coli K12 strain MG1655; (3) 在全基因组范围内检测准确的启动子活动; (4) 在质粒水平上,所挑选的DNA区域很容易突变,能够验证启动子区域cis-regulatory位点。 大肠杆菌标记ORFs文库 (E. coli Tagged ORFs) ...