后续他们又在E. coli MG1655中分别缺失这7个基因进行验证,结果证明吩噻嗪类药物确实是会抑制细胞能量代谢,影响细胞膜完整性。 更多推荐:
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靶向基因均被卡那霉素抗性盒取代，并带有可被FLP重组酶识别并切除的FRT位点。文库背景菌株为E. coli K-12 BW25113。研发时，共靶向4288个基因，其中303个为必需基因，包括37个未知功能基因，未能成功缺失。3985个基因成功缺失，其中3912个基因在MG1655和W3110均有注释，73个基因在先前研究中有注释。
Dihydrolipoamide dehydrogenase mutation alters the NADH sensitivity of pyruvate dehydrogenase complex of Escherichia coli K-12. Under anaerobic growth conditions, an active pyruvate dehydrogenase (PDH) is expected to create a redox imbalance in wild-type Escherichia coli due to incr... Youngnyun,Kim,L...
coli K-12 in having an extra ~25% of genes that are scattered over the entire chromosome. Therefore the work was also aimed to reveal the extent to which new members have joined the regulon. To compare Lrp regulon structures in two organisms, transcript profiles under the same growth ...
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coli K-12 : Inhibition of "Nitrogen mustard after-effect" and stimulation of gene... A.: Two kinds of activity promoted by culture filtrates of male strains of E. coli K 12: inhibition of “nitrogen mustard after-effect” and ... DM Goldfarb,LS Chernin,GI Goldberg,... - 《Molecular...
coli K-12 genome. Mehta P, Casjens S, Krishnaswamy S: Analysis of the lambdoid prophage element e14 in the E. coli K-12 genome. BMC Micro- biol 2004, 4:4... Mehta,Preeti,Casjens,... - 《Bmc Microbiology》 被引量: 80发表: 2004年 Shiga toxin-encoding bacteriophages--genomes in mot...