In their recent study, Link and colleagues (Schramm et al 2023) use a CRISPR‐based approach to systematically create TS alleles across essential genes in E. coli.Kumar, ArunStirling, Peter CMolecular Systems Biology
在此基础上,并结合在E.coli中基因转录与环境压力间的相关性以及自发单链断裂在静止期E.coli基因组中的普遍性,S·M·罗森博格等人推断:在E.coli承受环境压力并处于静止期时,R环可在一定程度上将DSB,进而将依赖于DSBR的适应性遗传变化,限制于基因组中与细胞对环境压力的适应较为相关的区域——转录基因和调控元件...
coli chromosome by using plasmid pKO3, a gene replacement vector that contains a temperature-sensitive origin of replication and markers for positive and negative selection for chromosomal integration and excision. Using kanamycin resistance (Knr) insertional alleles of the essential genes pepM and rpsB...
RpoE(σ24)是E.coli拥有的七个转录调控因子之一,亦是E.coli的一个基本转录调控因子,为E.coli在所有检测条件(如高温、低温、对数期、静止期)下的生存所必需(1)(2)。RpoE可在E.coli细胞进入静止期或面临热击、冷击、高渗、氧化、碳饥饿等多种环境压力时,通过调控被膜压力反应影响(上调或下调)包括rpoH和greA...
Genes regulating membrane-associated E-cadherin and proliferation in adenomatous polyposis coli mutant colon cancer cells: High content siRNA screen Among the genes whose depletion promotes membrane-associated E-cadherin, we identified ZEB1, the microRNA200 family, and proteins such as a ubiquitin ......
“I think it’s a tour de force, one of the top 10 papers of the year,” saysFrederick Blattnerof the University of Wisconsin-Madison, who was not involved in the study. “Even though the genes are essential, they can be altered.” ...
coli as host for the functional screening of large collections of proteins and peptides. Among the cutting-edge techniques demonstrated are those for rapid high-level expression and purification of soluble and functional recombinant protein and those essential to functional genomics, proteomics, and ...
These results indicated that these mutations likely changed the transcription of other genes thereby causing the enhancement of isopropanol and isobutanol tolerance in E. coli strains, as will be discussed more below. Since enhanced tolerance does not always lead to enhanced production, we introduced ...
摘要: An improved medium for the direct, positive selection of tetracycline-sensitive clones from a population of tetracycline-resistant strains of Escherichia coli is described.关键词: Escherichia coli Tetracycline Culture Media Drug Resistance, Microbial R Factors Selection, Genetic ...
(1)大肠杆菌Keio基因敲除文库(E.coli Keio Knockout Collection) 大肠杆菌Keio基因敲除文库包含一组大肠杆菌K-12中所有非必需基因的单基因敲除突变体。共计3985个基因,每个基因有两个独立的缺失菌株,共产生7970个菌株;每一个被删除的基因被一个卡那霉素抗性盒所取代,该盒侧翼带有FRT位点可被FLP重组酶识别并切除。