E. coli codon usage master table.Julius, B. Lucks
coli, only 16% of these proteins were expressed in soluble form [30]. Similar results were obtained with C. elegans proteins: 15% of the selected proteins were soluble [29]. Small numbers of soluble eukaryotic proteins are not surprising and could reflect problems with different codon usage ...
3a) segregated according to the identity of the nucleotide at each of the three positions in the codon. d–h, The codon slopes from model M plotted versus the relative synonymous codon usage (RSCU) in E. coli BL21 (e), the codon adaptation index14 in E. coli K12 (f), the codon ...
coli cells harboring this truncated DEBS, along with plasmids responsible for mycarosyl and desosaminyl sugar biosynthesis and transfer, are able to synthesize 6-deoxyerythromycin D (3) or its analog (4) when fed with an appropriate diketide analog precursor (1 or 2). Table 1 Plasmids used...
spartinae, the coding region was codon-optimized and synthesized de novo to replace the original NAT gene, using the restriction endonucleases NcoI and SphI. The resulting plasmid was named pUG72-NATss. Table 1 Primers used in this study Full size table To construct a gene deletion cassette...
coli strains used and generated in this study can be found in the key resources table. Method details Bacterial culturing Cells were grown in liquid or on solid LB medium or M9t medium (1x M9 salts, 0.1 mM CaCl2, 2 mM MgSO4, 2% (w/v) glucose, 0.5% tryptone, and 0.01% (w/v) ...
GlnR is an atypical response regulator found in actinomycetes that modulates the transcription of genes in response to changes in nitrogen availability. We applied a global in vivo approach to identify the GlnR regulon of Streptomyces venezuelae, which,
Codon usage is one of the factors influencing recombinant protein expression. We were interested in the codon usage of an antibody Fab fragment gene exhibiting extreme toxicity in the E. coli host. The toxic synthetic human Fab gene contained domains opt
coli preferred usage codons. Oligonucleotides 909-34 through 909-53 (See Table 1) were designed and used to construct the gene, and the complete gene sequence is depicted in FIG. 1. The oligonucleotides were designed to include an NdeI site at the 5' end of the gene and a BamHI site ...
Genes of interest were amplified by PCR using tailed primers (see Table S1 in the supplemental material). Each fragment was digested with the designed restriction enzyme, ligated into pETBlue-2, and transformed into E. coli Tuner or E. coli DH5α cells. A 50-ml culture of each transforman...