Escherichia coliRNA, BacterialRNA, Ribosomal, 16SRecombinant ProteinsDNA Mutational AnalysisBase SequenceNucleic Acid ConformationMutationPlasmidsMolecular Sequence DataA series of base substitution and deletion mutations were constructed in the highly conserved 530 stem and loop region of E. coli 16S rRNA ...
从印度西孟加拉邦 Kolaghat 水产养殖场采集自然感染濒死和未感染的印度鲥鱼样本,对鱼体组织进行处理,分离其中的真菌和细菌;二是分子生物学技术,通过 PCR 扩增真菌的 ITS 1 和 ITS 4 基因并测序,利用 16S rRNA 测序分析细菌种类,还进行了 qPCR 分析宿主免疫基因的表达;三是构建系统发育树,基于测序结果使用 MEGA X...
Design and identification of antisense oligonucleotide targeting to 16S rRNA of E. coli大肠杆菌16S rRNA的核酶设计和初步应用RNA反义核酸位点大肠杆菌16SRRNATargeting rRNA of bacteria is a new strategy for antibiotic agent development. The rRNA such as mRNA are naturally self-folded molecules which ...
coli regulatory network at an amino acid level. Regulatory network engineering for C3–C4 alcohol tolerance Higher-carbon alcohols can be blended with gasoline at a higher volume: ethanol is blended at 10%, whereas isopropanol or isobutanol can be blended at 16%. These higher volumes would lead...
At the nonpermissive temperature, this strain accumulates a novel "19S" RNA species which contains 17S precursor rRNA sequences covalently linked to tRNA sequences transcribed from the ribosomal RNA spacer region between the 16S and the 23S rRNA cistrons. In vitro treatment of 19S RNA with cell...
Related to E. coli:E. coli infection Escherichia coli [‚esh·ə′rik·ē·ə ′kō‚lī] (microbiology) The type species of the genus, occurring as part of the normal intestinal flora in vertebrates. Also known as colon bacillus. ...
大肠杆菌DNA连接酶(E. coli DNA Ligase)产品及特点: 本酶催化相邻DNA链的5′-P末端和3′-OH末端以磷酸二酯键结合的反应,需NAD作辅酶。本酶只能催化突出末端DNA之间的连接,但如果在PEG及高浓度一价阳离子存在的条件下,也能连接平滑末端的DNA,但不能催化DNA的5′-P末端与RNA的3′-OH末端以及RNA之间的连接。
coli. FlhG/FleN are ATPases and homologous to MinD. FlhG is structurally and functionally similar to MinD (20). Like MinD, FlhG forms a homodimer in the presence of ATP, tethers to the membrane with the help of conserved membrane-targeting sequence, and forms foci at both the poles. ...
The full-length 16S rRNA gene sequences of the 33 MCRPEC strains were used to generate a phylogenetic tree by means of Neighbor Joining method in MEGA-X (Fig.1). Thirty-three MCRPEC strains were classified into eight distinct STs, including ST10, ST224, ST361, ST410, ST641, ST1408,...
Evolutionary distance tree, based on 16S rDNA sequences, showing the relationship of F. tularensis with other putative members of the Francisellaceae and. Summary of major events in Earth history relating to the appearance of eukaryotic groups and the appearance of O2 in the atmosphere and in ma...