DZNep reduces EZH2 protein expression and H3K27me3 and but not mRNA.Nicolas GirardCéline BazilleEva LhuissierHervé BenateauAntonio LlombartBoschKarim BoumedieneCatherine Bauge
Cheng, LinZhang, JunjunYan, NaihongChen, DanianCell Death DisCell Death & DiseaseZheng S, Xiao L, Liu Y, Wang Y, Cheng L, Zhang J, Yan N and Chen D. DZNep inhibits H3K27- me3 deposition and delays retinal degenera- tion in the rd1 mice. Cell Death Dis 2018; 9: 310....
Western blot检测结果显示,与阴性对照组相比,实验组M23和SP6.5细胞中的EZH2(t=5.214,P=0.035;t=13.530,P=0.005)、p-Rb(t=4.551,P=0.045;t=4.655,P=0.043)、E2F1(t=8.090,P=0.015;t=9.313, P=0.011)以及p-ERK(t=4.819,P=0.040;t=8.951,P=0.01...
Western blotp-JNK / JNK / p-p38 / p38 / p-ERK / ERK / p-IκB-α / IκB-αHOXA6 / HOXB3 / HOXC4 / HOXD13EED / p21 / p-RB / p27 / p-CDC2TGFBR1 / TGFBR2 / Smad3Suz12 / H3K4me3 / H3K36me2EZH2 / H3K27me3
DZNep是一种组蛋白甲基转移酶抑制剂(histone methytransferase inhibitor, HMTi)[3],主要通过降低EZH2的活性并诱导H3K27me3降解,从而抑制下游靶基因的转录阻碍肿瘤的进展,这也是DZNep抗肿瘤活性的主要途径。DZNep可有效诱导组蛋白甲基转移酶成员-核受体结合SET结构域蛋白2(nuclear receptor binding SET domain containing...
Western blotEZH2 / H3K27me3Suz12 / H3K4me3 / H3K36me2TGFBR1 / TGFBR2 / Smad3EED / p21 / p-RB / p27 / p-CDC2HOXA6 / HOXB3 / HOXC4 / HOXD13p-JNK / JNK / p-p38 / p38 / p-ERK / ERK / p-IκB-α / IκB-α
( C川、细胞的增殖能力;流式细胞术检坝 细胞凋亡;平板 隆法检坝 细胞形成克隆能力;JC- 荧光探针染色法检测细胞线粒体膜电位;蛋白质印 法(WB)检 EZH2 细胞增殖核抗原(Ki 67(CCASP 3)的表达体内实验裸鼠皮下荷揭模型 通过免疫组织化学染色及 位才端标记(TUNE )法检测1χN�P对细胞增殖、凋亡的作用 果:...
外泌体,细胞间连接,上皮-间充质转化背景与目的:乳腺癌是全球女性发病率最高的恶性肿瘤,患者预后差.肿瘤源性外泌体会改变肿瘤微环境并参与调控肿瘤的发生,发展及转移,这将为肿瘤的诊断和治疗提供新的思路.DZNep能够靶向调控H3K27me3组蛋白甲基转移酶的降解,并特异性地诱导肿瘤细胞凋亡,从而抑制多种肿瘤细胞增殖和...
In this study, we found that trimethylation of several lysine sites of histone H3, including lysine 27 (H3K27me3), increased in the retinas ofrd1mice. Histone methylation inhibitor DZNep significantly reduced the calpain activity, delayed the photoreceptor loss, and improved ERG response ofrd1...
H3K27me3NMDANeuroprotectiveRetinal ganglion cellsEpigenetic gene enhancer of zeste homolog-2 (Ezh2) is reported to be associated with ocular neurodegenerative diseases; however, its underlying mechanism is poorly understood. The present study aimed to determine the role of 3-deazaneplanocin A (DZNep)...