Whacked and Rab35 polarize dynein-motor-complex- dependent seamless tube growth. Nature cell biology 2012;14:386-93.Schottenfeld-Roames, J. and Ghabrial, A. S. (2012). Whacked and Rab35 polarize dynein-motor-complex-dependent seamless tube growth. Nat. Cell Biol. 14, 386-393....
Moreover, since the intermediate chains are at the base of the dynein complex in the cargo-binding region, which is attached to the A-tubule of the doublet microtubule, we hypothesized that the binding partner in the 200-kDa crosslinked product is tubulin. We took additional steps to confirm...
a ubiquitous minus end-directed microtubule-based motor protein, is believed to be responsible for the retrograde transport of membranous organelles from the synapse to the cell body in fast axonal transport. The cytoplasmic dynein complex is composed of two ~530 kDa heavy chains, and intermediate ...
Binding of KIF1C releases the HOOK3 autoinhibited folded conformation allowing dynein to bind the adapter. In this cocomplex, KIF1C further acts as a processivity factor for dynein. Ferdos Abid Ali Alexander J. Zwetsloot Anne Straube ResearchOpen Access02 Jan 2025 Nature Structural & Molecular...
Movement of cargo towards the minus-end of microtubules is performed by cytoplasmic dynein-1 that is in a complex with the regulatory factor dynactin and cargo adaptor proteins(reviewed in 1). Mutations in dynein, its regulatory proteins, and cargo adaptor proteins have all been associated with ...
We used affinity chromatography to probe for a direct binding interaction between cytoplasmic dynein and dynactin. Purified cytoplasmic dynein was found to bind to an affinity column of p150Glued, the largest polypeptide in the dynactin complex. To test the specificity of the interaction, we loaded ...
Dynactin1 is the largest subunit of the dynactin complex [23] and belongs to the microtubule plus end-binding protein family [24]. The microtubule-binding CAP-Gly (cytoskeleton-associated protein-glycine-rich) domain is located at the N-terminus [23,25]. The CAP-Gly domain is connected to ...
Dynactin1 is the largest subunit of the dynactin complex [23] and belongs to the microtubule plus end-binding protein family [24]. The microtubule-binding CAP-Gly (cytoskeleton-associated protein-glycine-rich) domain is located at the N-terminus [23,25]. The CAP-Gly domain is connected to ...
Dynactin is a multisubunit protein complex that is required for most, if not all, types of cytoplasmic dynein activity in eukaryotes. Dynactin binds dynein directly and allows the motor to traverse the microtubule lattice over long distances. A singlDynactin is a multisubunit protein complex that...
Arl8b, a small GTP-binding (G) protein, promotes lysosome anterograde trafficking mediated by kinesin-1. Herein, we report an Arl8b effector, RUFY3, which regulates the retrograde transport of lysosomes. We show that RUFY3 interacts with the JIP4-dynein-dynactin complex and facilitates Arl8b ...