2× 106 cells were harvested, washed once in PBS and lysed for 30 min in ice with RIPA buffer (50 mM Tris, pH8, 1% NP-40, 0.5% DOC, 0.1% SDS) containing protease inhibitors (Complete, Roche) and 1 mM PMSF. After centrifugation (14000 rpm, 20 min, 4°C) the supernatant was ben...
The cells were fixed with 4% paraformalde- hyde for 10 min on ice, permeabilized in 1% v/v Triton-X 100, washed with PhosFlow Perm/Wash Buffer I (BD Biosciences, San Jose, CA, USA), and resuspended in a buffer. Antibodies PE anti-nestin (BDB561230), PerCP-Cy5.5 anti-SOX1 (BDB...
According to their algorithm, the predicted dynamics were fitted first, then a trajectory was sampled from the replay buffer to update the actor network, after which an imagined future transition was obtained and was used to update the critic network. Both of the MB and MF parts were used to...