A new metric has recently been promoted by Illumina Corporation called the DV 200. As noted in a published tech note from Illumina Corporation 2 “Many researchers use the Agilent RNA Integrity Number (RIN) to determine RNA quality for gene expression analysis. However we have found that RIN ...
Quickly determine the quality of your FFPE RNA samples with the Automated Electrophoresis Portfolio TheAutomated Electrophoresis systemsfrom Agilent utilize a quality metric, DV200, to address the unique QC challenges of FFPE RNA. The DV200provides researchers with a tool to reliably classify degraded ...
采用 Agilent RNA ScreenTape 分析法获得的 RNA 完整值当量 (RINe) 是一种评估标准 RNA 测序的样品完整性的可靠且可重现的指标。由于 RNA 降解以及样品体积有限,从福尔马林固定石蜡包埋 (FFPE) 组织中提取 RNA 样品进行文库制备具有挑战性,因此建议针对 FFPE RNA 文库制备采用定制方案。FFPE RNA 样品的片段大小分布...
RNAScreenTape分析法有助于对从FFPE组织中提取的降解RNA样品进行高可重 现的DV 200 评估。AgilentTapeStation分析软件能够通过区域分析对DV 200 数据进行 简单评估和处理,自动进行重复DV 200 分析。 采用RNAScreenTape分析法进行 DV 200 评估 2 技术详情 使用配有AgilentTapeStation分析软件 ...
对于未能与内标有效分离的高度降解样品,应对其进行稀释以获得最准确的结果。 参考文献 1. Evaluating RNA Quality from FFPE Samples.Illumina Technical Note, publication number 470-2014-001.https://www.illumina.com/ documents/products/technotes/ technote- truseq-rna-access.pdf...
DV200RNA Nano 和 DV200RNA Pico 分析是实用工具,能够自动定义从 FFPE 组织中提取的降解 RNA 样本的 DV200区域。分析被导入后,即可用于运行Agilent 2100 生物分析仪RNA 芯片或重新分析现有数据文件。DV200结果可保存、导出并显示在报告中。 参考文献 1. Evaluating RNA Quality from FFPE Samples. Illumina,Technica...
Evaluation of RNA quality has traditionally relied upon the RNA integrity number (RIN). Developed by Agilent, the RIN algorithm measures the integrity of total RNA and assigns a 1 to 10 score; 10 being the most intact and 1 the most degraded. In general RINs of 7-8 are the gold ...
全部▾ Quickly determine the quality of your FFPE RNA samples with the Automated Electrophoresis Portfolio TheAutomated Electrophoresis systemsfrom Agilent utilize a quality metric, DV200, to address the unique QC challenges of FFPE RNA. The DV200provides researchers with a tool to reliably classify...
DV200RNA Nano 和 DV200RNA Pico 分析是实用工具,能够自动定义从 FFPE 组织中提取的降解 RNA 样本的 DV200区域。分析被导入后,即可用于运行Agilent 2100 生物分析仪RNA 芯片或重新分析现有数据文件。DV200结果可保存、导出并显示在报告中。 参考文献 1. Evaluating RNA Quality from FFPE Samples. Illumina,Technica...
Quickly determine the quality of your FFPE RNA samples with the Automated Electrophoresis Portfolio The Automated Electrophoresis systems from Agilent utilize a quality metric, DV200, to address the unique QC challenges of FFPE RNA. The DV200 provides researchers with a tool to reliably classify degra...