The complex formed by ORC-Cdc6 bound to duplex DNA (OC) recruits the MCM-Cdt1 complex into the replication origins. Through the stacking of both complexes, the duplex DNA is inserted inside the helicase by an unknown mechanism. In this paper we show that the DNA insertion comes with a ...
Nucleosome assembly during DNA replication occurs in a stepwise fashion. Early studies using a chemical cross-linking technique combined with radioisotope labeling methods demonstrated that parental histone H3–H4, which is one of the major carriers of epigenetic information, needs to be recycled during ...
It is therefore uncertain whether gene expression and chromatin state drive chromatin organization or whether changes in chromatin organization facilitate cell-type-specific activation of gene expression. Here, using the dorsoventral patterning of the Drosophila melanogaster embryo as a model system, we ...
Transcription factors (TFs) interpret the genome directly, and are responsible for decoding DNA sequences [1]. It is reported that transcriptional factors are key components of cells that control gene expression, determining how the cells function [2]. Transcription factors acting as conductor orchest...
By performing high-depth rRNA depletion RNA-Seq and investigating genome-wide chromatin accessibility, DNA methylation, and histone modification, we identified thousands of transcripts corresponding to enhancer regions in Arabidopsis and revealed that the formation of a “transcription hub” is the critical...
Relative transcription levels of AchnbZIP29 and AchnMYB70 during wound suberization of kiwifruit. “**” represents significant difference at p ≤ 0.01 Full size image Discussion Abscisic acid (ABA) is a stress resistance hormone in plant, which is involved in a variety of biotic and abio...
regulation of transcription, DNA-templated, and response to abscisic acid were the most enriched pathways, whereas in the LT-vs-Cont group, the DEGs were mostly associated with translation, response to salt stress, and response to cold in the biological process category. In the cellular component...
In our simulations (Supplementary Movies 1–21), for some of the studied loci (Sox2, Mmp12) we observed a “caging” effect of the transcription start site (TSS) at the time when the locus was transcriptionally active. To quantify this observation, we first calculated for each TSS the time...
Cell division is the basis for the propagation of life and requires accurate duplication of all genetic information. DNA damage created during replication (replication stress) is a major cause of cancer, premature aging and a spectrum of other human diso
In all Metazoa, transcription is inactive during the first mitotic cycles after fertilisation. In Drosophila melanogaster, Zygotic Genome Activation (ZGA) occurs in two waves, starting respectively at mitotic cycles 8 (approximately 60 genes) and 14 (ove