The complex formed by ORC-Cdc6 bound to duplex DNA (OC) recruits the MCM-Cdt1 complex into the replication origins. Through the stacking of both complexes, the duplex DNA is inserted inside the helicase by an unknown mechanism. In this paper we show that the DNA insertion comes with a ...
What are the genetic components of an operon and how do they control transcription? What is another term for DNA synthesis? What affects the rate of transcription? What is made during DNA replication? What is a possible effect of an error during DNA transcription?
1a). However, only the tonic mvimT terminal expressed the HB9 transcription factor (Supplementary Fig. 1a). As is typical of Drosophila synapses19, the mvimT presynaptic terminal is composed of a chain of linked bouton varicosities each of which contains multiple active zones for vesicle ...
(A) AnE. coliRNA polymerase (RNAP) elongation complex is tethered via a pair of double-stranded DNA handles and held on the optical tweezers. Active transcription at different [NTP] is monitored in real time, where the growth and folding of nascent RNA is detected as the tether extension c...
(Figure4E) showed that theDIPMtranscript and DipM protein levels change through the cell cycle progression and peak during the S phase, as doMINDandMINE[32]. Thus, DipM transcription and translation are regulated by the cell cycle such that DipM is specifically expressed during chloroplast ...
Exposure to cold temperatures and exercise is known to increase browning (7), and there are several factors that specifically control the process (5, 8, 9). For instance, transcription factors such as peroxisome proliferator-activated receptor gamma (PPARγ) and CCAAT-enhancer-binding proteins (C...
By performing high-depth rRNA depletion RNA-Seq and investigating genome-wide chromatin accessibility, DNA methylation, and histone modification, we identified thousands of transcripts corresponding to enhancer regions in Arabidopsis and revealed that the formation of a “transcription hub” is the critical...
In all Metazoa, transcription is inactive during the first mitotic cycles after fertilisation. In Drosophila melanogaster, Zygotic Genome Activation (ZGA) occurs in two waves, starting respectively at mitotic cycles 8 (approximately 60 genes) and 14 (ove
EGR2 (members of the early growth response family) ist among the prominent genes of the intermediate response metagene M2. This transcription factor is the target of NFAT transcription factors [37,38], acts as a transcriptional repressor, and impairs IL2 transcription in anergy [39]. IL2 is ...
It has been reported that DNA methylation can repress gene expression [56]. However, the relationship between DNA methylation and gene transcription is more nuanced than initially realized. For example, rice promoter methylation repressed gene expression only in some heavily methylated gene loci; on ...