For example, at D20 the first stage of glycolysis that utilizes ATP to store or release glucose is enhanced, while at D4, the ATP-generating phase is enhanced to provide energy for rapid cellular proliferation at this time point. This work has also identified several metabolites, including ...
At early stages of the exponential growth phase in HEK293 cell cultures, the tricarboxylic acid cycle is unable to process all the amount of NADH generated in the glycolysis pathway, being lactate the main by鈥恜roduct. However, HEK293 cells are also able to metabolize lactate depending on ...
By labelling pollen cultures with 32P-orthophosphate, it has been possible to quantitate the nucleotide components of the pollen, and thereby to measure changes in the nucleotide pattern at various growth stages. The most marked changes occur during the initial phase of tube growth when a large in...
In animal andin vitromodels, it has been demonstrated that differentiated ECs are characterized by glycolytic formation of adenosine 5′-triphosphate (ATP) for energy production and have mitochondrial respiration as the secondary source of ATP. It has been shown that ECs increase glycolysis in respons...
Mammalian follicle development is characterized by extensive changes in morphology, endocrine responsiveness, and function, providing the optimum environment for oocyte growth, development, and resumption of meiosis. In cattle, the first signs of transcr
Naked mole-rats are among the most hypoxia-tolerant mammals. During hypoxia, their body temperature (Tb) decreases via unknown mechanisms to conserve energy. In small mammals, non-shivering thermogenesis in brown adipose tissue (BAT) is critical to Tb re
we addressed the above question by monitoring the biomass changes and the N and C budget during somatic embryo germination, under low-intensity red light. We found that the C and N storage reserves, accumulated during the maturation phase, were not sufficient to support the growth of the germin...
Several connectome-transcriptome association studies have demonstrated a nexus between functional connectivity in the brain and the expression levels of genes enriched for ion channel activity, synaptic function, and aerobic glycolysis [26], [27]. Thus, we speculate that the developmental changes in ...
Briefly, cells were grown at 25 °C (permissive temperature) to a cell density of 6–7 × 106 cells/ml and arrested at late G2 phase by shifting to 36 °C (restrictive temperature) for 5 h. Cells were released into the cell cycle by shifting the temperature back to 25 °C. The ...
The prevailing chronic hypoxic conditions at HA begin to negatively affect intrauterine growth of the human fetus at the end of the second trimester10, a time window overlapping with the main phase of nephrogenesis. The kidneys of such SGA infants are characterized by a lower number of nephrons...