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The extracted DNA was used as a template to amplify the region encompassing ITS-1, 5.8S rRNA, ITS-2, and the D1/D2 domains of the 28S (LSU) rRNA gene using the primers ITS5F (5 -GGAAGTAAAAGTCGTAACAAGG-3 ) and NL4R (5 -GGTCCGTGTTTCAAGACGG-3 ) [14,15] with the following...
(Color figure online) qPCR and breakpoint analysis Fusion transcript analysis First, the qPCR assay was carried out with a set of five primer pairs in the proband and his mother, in whom it confirmed the array CGH results. Next, other rounds of qPCR done in the proband allowed to narrow ...