Die Technologie Duolink<sup>®</sup>-Proximity-Ligation-Assay (PLA) für den Nachweis und die Quantifizierung von Protein-Protein-Interaktionen.
Analysis is carried out using standard immunofluorescence assay equipment.SpecificityDuolink®In Situfluorescence applications use two wash buffers. Wash Buffer A is used after the PLA Probe incubation step and Wash Buffer B is used after incubation with the amplification reagents. See datasheet for ...
价格 2849.86元*本价格含增值税费 服务 √原厂保证 √包邮 √增值税票 数量 -+ QQ微信 添加到订购单查看订购单 产品名称: Duolink®flowPLA Compensation Beads – FarRed 产品介绍: 产品说明 应用 Duolink®proximity ligation assay(PLA®) allows for endogenous detection of protein interactions, post ...
产品性质 产品线 Duolink® technique(s) flow cytometry: suitableimmunofluorescence: suitableproximity ligation assay: suitable 荧光 λex 390 nm; λem 476 nm 适用性 suitable for fluorescence 运输 dry ice 储存温度 −20℃ 安全信息 储存分类代码 10 - Combustible liquids WGK WGK 2Sigma...
For the Proximity Ligation Assay (PLA). Sensitive, in-situ detection of endogenous proteins, protein modifications, and protein-protein interactions.
technique(s)flow cytometry: suitable immunofluorescence: suitable proximity ligation assay: suitable 荧光λex390 nm; λem476 nm 适用性suitable for fluorescence 运输dry ice 储存温度−20℃ 安全信息 储存分类代码10 - Combustible liquids WGKWGK 2
Duolink PLA (Proximity Ligation Assay)是一种用于检测蛋白质互作的荧光实验技术。该技术使用特定的荧光标记探针来检测两个靠近的蛋白质分子之间的相互作用。 实验步骤如下: 1.细胞处理:首先,处理您要研究的细胞。您可以使用适用于您研究的细胞类型的特定处理方法。这可能包括对细胞进行激活、转染或处理以诱导互作事件...
proximity ligation assay: suitable fluorescence【荧光】 λex 594 nm; λem 624 nm (Texas Red®, Zeiss Filter set 31) suitability【适用性】 suitable for fluorescence shipped in【运输】 dry ice storage temp.【储存温度】 −20℃ 安全信息 Storage Class Code【储存分类代码】 10 - Combustible liqui...
与传统的免疫测定相比,Duolink提供了一种简单、灵敏度高的原位检测内源蛋白的方法。该实验基于邻位连接技术(Proximity Ligation Assay, PLA),当一对PLA 探针足够接近(<40 nm)时会产生PLA信号,PLA信号的位置直接反映了蛋白表达量或蛋白复合体的亚细胞定位。
PLA probes. Analysis is carried out using standard immunofluorescence assay equipment. Specificity Red fluorescence detection reagents are often used with Texas Red?filter. Application Note Two primary antibodies raised in different species are needed. Test your primary antibodies (IgG-class, mono- or ...