and cultured them to generate liverorganoids. Our data demonstrated that these organoids express hepatocyte marker FAH when transplanted in vivo, suggesting that duct cells from biliary tree have both proliferative and differentiation capability. These data suggested that once the clonogenic cholangiocyte...
The purities of the obtained ductal cells were approximately 98% in humans and 93% in rats. Furthermore, intracellular free calcium increased with time and concentration of carbachol treatment. Cytokeratin 19 serves as a dependable marker for identifying ductal cells in salivary glands, except for ...
The normal breast microenvironment consists of ductal cells separated by a myoepithelial layer from the immune cells and fibroblasts residing within a collagen-rich extracellular matrix [44]. With malignant transformation, the myoepithelial barrier breaks down and interaction between these cell types and ...
S3. Black arrow = band of interest; MWM = molecular weight marker. (C) Expression of ZsGreen1 (a green fluorescent protein variant) in primary epithelial cells and SV40T immortalized cells transduced with the LV-GK; phase and inverted fluorescent microscopy of living cells. Measure ...
(red) and nuclear marker (blue).EtoH: only SGLT1 in green color. The SGLT1 protein in ductal cells of WKY can be seen in a very low intensity (AandE), whereas the SGLT1 immunoreactivity is clearly observed in WKY-D (BandF) and SHR (CandG); a further increase in SGLT1 can be ...
The potential of PDC as tumor-originating cells, however, remains controversial in contrast to accumulating evidence that HCC originates from hepatocytes. Here, we demonstrate that PDCs expressing the established surface and cancer stem cell marker EpCAM give rise to HCC in inflamed liver. EpCAM-...
The identification of ductal plate cells as likely progenitors for bile duct epithelium and hepatocytes and their possible reappearance as oval cells in the regenerating liver have generated much interest in their pluri-potential capacities. We have examined the distribution of three hematopoietic stem ce...
Cultured cells, as well as ductal epithelial cells in pancreatic tissue, showed nuclear localization of the transcription factor SOX9 (Fig. 3B), and stained positive for the ductal epithelial marker keratin 7 (KRT7; Fig. 3C). Fig. 3 Pancreatic organoid-derived cells form a polarized...
The histochemical staining of granular ducts by DMAB- nitrite showed loss of kallikrein, which is a marker for ductal function,13 secretory granules of granular tubule cells 3 days post duct ligation (Figure 6d) in comparison with unoperated controls (Figure 6c), rapamycin treatment following ...
that was associated with (1) the maintenance of immunoreactivity for neuron-specific enolase, an endocrine cell marker, but a progressive loss of insulin gene expression, (2) a loss of immunoreactivity for insulin protein, and (3) the appearance of CK-19, a marker for ductal cells. After...