cDNA was diluted (1:10) and the one step PCR reaction was performed in a total volume of 20 µL using the GoTaq qPCR Master Mix (Promega) according to the manufacturer’s protocol. The PCR condition was set at an initial denaturation at 95 °C for 10 min, 45 cycles at 95 °C ...
Data are means ± SEM of at least three independent experiments performed in duplicate. * p < 0.05; ** p < 0.01. To test if L30I40 induces growth inhibition and cell apoptosis through regulating cell-cycle-related proteins and apoptosis effectors in MCF7 and T47D, we measured master ...