Finally, samples were quantified using the Bioanalyzer High-Sensitivity DNA assay (Agilent Technologies) and sequenced using the Novaseq 6000 platform. Dual-luciferase reporter assay The dual-luciferase reporter assay was performed as previously described39. Briefly, the 3′-UTR of human OOSP2 was ...
Cells were assayed with a Dual-Luciferase Reporter Assay System (Promega). Statistical analysis of data Data are presented as the mean ± standard deviation determined from at least three independent experiments. Differences were assessed by two-tailed Student’s t-test using Excel software (...
(K) IFN-β luciferase expression in ISG-THP1 cells after indicated treatments. Data are represented as mean ± SEM,n= 3. Two-tail t test in (C) and one-way ANOVA followed by Dunnett’s test in (D) and (K). ∗:p< 0.05, ∗∗:p< 0.01, ∗∗∗:p< 0.001. ...
thymidine kinase (TK) promoters, and test gene activities using two BL signals. Perhaps the most popular format, typified by the Promega Dual-Luciferase®Reporter (DLR®) assay system5, involves two unrelated luciferases that are assayed sequentially without filters using two substrates and a Lu...
luciferase activity) represent a surrogate function readout of ligand-receptor interaction. With that, we first tested HX009 in a reporter assay for PD1 blocking function, where Jurkat cell line was engineered to carry an IL-2 promoter-guided luciferase reporter gene36, and the luciferase signal ...
and the activities of firefly and Renilla luciferase were measured using the Dual-Luciferase Reporter Assay System (Promega, Cat# E1960) and Luminoskan Ascent machine (Thermo Scientific). Renilla luciferase values were normalized to firefly luciferase activity to obtain the relative luciferase activity ...
12 h after transfection with the TOPFLASH reporter plasmid construct, cells were incubated with Wnt3a or control conditioned medium for 6 h. Cells were then incubated with Dkk1/2-GFP conditioned medium as indicated for another 12 h before harvest of cell lysates for the luciferase assay. Bars ...
(hp32) at the indicated E:T ratios. Eighteen hours later, the cytotoxic action of the human CAR T cells was measured by quantifying luciferase activity in tumor target cells. Data represent mean ± SEM.N = 5 for GBM andN = 4 for U87 independent experiments, two different ...
Luciferase assay Cells were transfected with pGL2-basic-CD47 or pGL2-basic-CD47-ΔNF-κB or pGL2-NF-κB luciferase reporters, and luciferase activity was measured by Luminometer (Promega, Madison, WI). For normalization of the reporter transfection efficiency, the total protein concentration of ly...
Using in vitro competitive binding assay, we demonstrated that GSK’074 bound to the kinase domain of recombinant human RIP3 with a Kd value of 130 nM (Fig.4a). Unexpectedly, GSK’074 also displayed significant binding affinity to RIP1 (Kd = 12 nM) (Fig.4b). In contrast, no...