Gey"s红细胞裂解液(Gey"s Lysis Buffer ) 100ml Gey"s红细胞裂解液(Gey"s Lysis Buffer ) 500ml 铬酸钾溶液(100g/L) 500ml HEPES缓冲液(50mM,pH=7.8) 100ml HEPES缓冲液(50mM,pH=7.8) 500ml 琥珀酸钠溶液(0.1M) 100ml 核酸沉淀溶液(TCA法) 100ml 核酸沉淀溶液(焦磷酸钠法) 100ml HRP标记抗体稀释...
C0403-100 通用Buffer Triton Lysis Buffer 100ml C0403-500 通用Buffer Triton Lysis Buffer 500ml R21908-15 通用Buffer Washing/Dilution Buffer 15ml R20613-100 通用Buffer XB盐溶液(20×) 100ml R20613-500 通用Buffer XB盐溶液(20×) 500ml T1630-250 通用Buffer 小分子10×电转缓冲液 250ml T1630-50...
1×CHAPS Lysis Buffer 100ml、500ml T16439-100 4×Tris-Hcl缓冲液(1.5M,pH=8.8) 100ml、500ml H1211-500 1M HCl 500ml H1210-500 1mM HCl 500ml T16406-500 20%盐酸 500ml R21824-500 1/10 N 盐酸 500ml R21962-1 1M DTT溶液/二硫苏糖醇 1ml、5ml R21982-1 ...
Half a million cells were harvested from six-well plates resuspended in lysis buffer containing 150 mM sodium chloride, 1.0% NP-40, 0.5% sodium deoxycholate, 0.1% SDS, and protease inhibitor cocktails (Complete protease inhibitor cocktail, Roche, Basel, Switzerland) and incubated on ice for 30...
laemmli lysis-buffer38733-5ml 去离子水38796-1l 酸性磷酸酶, 白细胞(trap) kit387a-1kt tyzorte 有机钛酸盐388173-100ml 水 质谱级39253-1l-r 茉莉酮酸甲酯392707-25ml 茉莉酮酸甲酯392707-5ml 碳二亚胺edc wsc39391-10ml 氨基胍 盐酸盐,甲脒肼盐酸盐396494-25g ...
C0403-100 通用Buffer Triton Lysis Buffer 100ml C0403-500 通用Buffer Triton Lysis Buffer 500ml R21908-15 通用Buffer Washing/Dilution Buffer 15ml R20613-100 通用Buffer XB盐溶液(20×) 100ml R20613-500 通用Buffer XB盐溶液(20×) 500ml T1630-250 通用Buffer 小分子10×电转缓冲液 250ml T1630-50...
C0721-500 常规溶液 1×CHAPS Lysis Buffer 500ml T16439-100 常规溶液 4×Tris-Hcl缓冲液(1.5M,pH=8.8) 100ml T16439-500 常规溶液 4×Tris-Hcl缓冲液(1.5M,pH=8.8) 500ml R21962-1 常规溶液 1M DTT溶液/二硫苏糖醇 1ml R21962-5 常规溶液 1M DTT溶液/二硫苏糖醇 5ml R21982-1 常规溶液 2M DTT...
Following incubation at 37°C in 5% CO2 for 18–20 hours, the cells were then washed twice with PBS and lysed with 50 μl cell lysis buffer (Promega, Madison, WI). The cell lysate (20 μl) was mixed with 90 μL of luciferase assay reagent (Promega) and measured on a Lumat LB ...
Following incubation at 37°C in 5% CO2 for 18–20 hours, the cells were then washed twice with PBS and lysed with 50 μl cell lysis buffer (Promega, Madison, WI). The cell lysate (20 μl) was mixed with 90 μL of luciferase assay reagent (Promega) and measured on a Lumat LB ...
Sperm samples from five different individuals for each species were included in the study. DNA decondensation included three lysis steps: first, a SDS + DTT incubation for 30 minutes; second, a DTT + NaCl treatment for 30 minutes; ... J Ribas-Maynou,E Garcia-Bonavila,M Llavanera,... ...