However, the leading FRET pair of this class (CFP/YFP) entails suboptimal donor excitation by Argon lasers, thereby hindering FRET imaging on many confocal microscopes. Further challenges arise from the large spectral overlap of CFP/YFP emission. By contrast, DsRed, along with other members of ...
0.4 ml fractions were collected from the bottom, sucrose concentrations determined, and fluorescence emission spectra of each fraction were obtained using a Hitachi F-2000 fluorescence spectrophotometer as described [14]. Excitation wavelength for DsRed was 558 nm, and for enhanced GFP 475 nm. Peaks...
The pigment geometry of DsRed was first examined by employing several model molecules whose spectra in gas-phase were actually observed, and an adequacy was confirmed. The excitation energy was then calculated to be 2.28 eV at the MLFMO-CIS(D)/6-31G level. The emission energy was also ...