One parameter may be antibody affinity, as theoretically only high affinity antibodies may bind in vivo in a stable way. This was analyzed in (NZB NZW)F1 mice with full-blown lupus nephritis. These mice had serum antibodies to dsDNA, and IgG autoantibodies bound in situ in glomerular membrane...
The study of SLE serum and monoclonal antibodies also demonstrated that not every antibody catalogued as anti-dsDNA or anti-DNase1L3 has dual reactivity to these antigens. There are a significant number of SLE sera that are only positive for anti-dsDNA or anti-DNase1L3 antibodies, not all a...
The antibody isotype can be IgG (IgG1, IgG2, IgG3, IgG4), IgM, IgA, IgE, or IgD. In some embodiments, the chimeric antibody comprises complementarity determining regions (CDRs) derived from a non-human animal. In some embodiments, chimeric antibody comprises a variable region derived from...
Autoantibodies against DNA are IgG type or IgM type reacting with natural double stranded DNA (dsDNA), single-stranded DNA (ssDNA), or both types. In human SLE (systemic lupus erythematosus), there found anti-dsDNA-IgG with high incidence. In SLE blood anti-dsDNA titer closely related to DNA...
1.3金标记羊抗人IgG 1.3.1亲合层析提纯羊抗人IgG为华美生物工程公司产品。 1.3.2胶体金溶液由本实验室参考全国临床检验操作规程[3]通过鞣酸.枸 橼酸钠还原法制备直径为5nm的胶体金溶液。 A液:1%HAuCl4水溶液1ml加入79ml双蒸馏水中混匀。 B液:1%枸橼酸三钠4ml,1%鞣酸0.7ml,0.1mol/LK2CO3液0.2ml, ...
Anti-H4-PK antibodies were detected in 56% SLE sera (more frequently in patients with skin or joint involvement) vs 12% disease controls; antibody titer is correlated with ECLAM score and anti-C1q antibodies, negatively with C3 levels. Anti-H4-PK antibodies compared with CLIFT and solid ...
Flu-specific antibody avidity was tested by ELISA. The levels of autoantibodies were analysed in plasma by ELISA Results Women had higher levels of all 3 IgG autoantibodies compared to men at D0, but not flu-specific neutralising activities nor higher flu-specific antibody avidities. The median ...
Afterwards, plates were washed with washing solution and incubated with alkaline phosphatase-conjugated goat anti-human IgG antibody (Thermo Fisher Scientific, Waltham, MA, USA) (80 μL/well, diluted 1:1000 in PBS-Ca) for 2 h. Following incubation with Nitro Blue Tetrazolium solution (Thermo ...