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To extract DNA from the 0.2-μm size-filtered cell and particulate fraction of soil, 250 μL of Phenol:Chloroform:Isoamyl (pH 8) was added to each sample and vortexed for 1 min. Samples were incubated on ice for 15 min, vortexing occasionally to homogenize samples, and centrifuged ...
sigma aldrich(西格玛) | Invitrogen(英骏) | Millipore | Abcam | CST | Roche | peprotech | prospec | pierce | BD Pharmingen | BD Biocoat | R&D | BioVision | Vector | Enzo life | eBioscience | ABR | Amresco | Cayman Chemical | 脂蛋白 | Merck | Qiagen(凯杰) | Santa cruz(三塔抗体...
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Santa Ana Winds of Southern California Impact PM2.5 with and without Smoke from Wildfires. GeoHealth 2020, 4, e2019GH000225. [Google Scholar] [CrossRef] Oakley, N.S. A Warming Climate Adds Complexity to Post-Fire Hydrologic Hazard Planning. Earth’s Future 2021, 9, e2021EF002149. [Google...
The collecting tube was placed in an ice bath until centrifugation (10 min, 1270 G, 4 ◦C). Approximately 100–150 µL of plasma was transferred to Nunc cryotubes (Thermo Scientific, Waltham, MA, USA) and frozen at –18 ◦C or below before analysis. 2.10. Analysis of Plasma ...
The cells were kept on ice until flow cytometry analysis was performed. The gating strategy used in this study was as follows: dead cells were excluded by gating infra-red dye negative cells, subsequently gated on the basis of forward scatter height versus forward scatter area (singlets 1), ...