h Bar graphs of (g). RAW264.7 macrophages were pre-treated with GA (100 µM) for 16 h followed by the stimulation of LPS (1 µg/mL) for another 8 h. HCECs were pre-treated with GA (100 µM) for 23 h followed by the stimulation of sodium chloride solution (90 mM) for ...
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The reactions were detected with streptavidin–phycoerythrin using a Luminex 100 IS 2.3 system (Austin, TX, USA). The limit of detection of this assay was 3.4 pg/mL for IFN-γ. 4.13. Statistical Analysis Between group mean differences between WT and Spdef −/− KO mice were compared by...