Retinal cells were dissociated using the Papain Dissociation System (Worthington Biochemical, Lakewood, NJ, USA) following the manufacturer’s instructions, and diluted to a final concentration of 1000 cells/µl. For each rd10 mouse group, three retinas (one from each of n = 3 female ...
Biochemical Corp. (Lakewood, NJ, USA). 2.2. Production of RHO-Loaded PLGA NPs PLGA was dissolved in dichloromethane/methanol (4:1w/w) solution at a polymer/solvent concentration of 0.5%w/v% and stirred at 300 revolutions per minute (rpm) for 12 h at room temperature (RT). To produce ...