Selection of compounds for testing in a mouse model of HAT. To explore anti-trypanosomal effects of the compounds in mice, we selected representatives of each class as defined in Fig. 1. Compound 1 was the best Class 1 candidate to test in vivo because it is well-tolerated in mice...
2.9. Drug Testing Process Flow Integration and Validation The drug assay was performed 7–10 days post-thawing in cells plated with traditional manual pipetting or bioprinting. The cells were loaded with the fluorescent dyes (Di-4- ANEPPS and Fura4F-AM, for voltage and intracellular calcium ...